Exogenous Salicylic Acid Ameliorates the Adverse Effects of Salt Stress on Antioxidant System in Rosmarinus officinalis L | ||
| Egyptian Journal of Botany | ||
| Article 9, Volume 58, Issue 2, July 2018, Pages 249-263 PDF (2.73 M) | ||
| Document Type: Regular issue (Original Article) | ||
| DOI: 10.21608/ejbo.2018.1772.1124 | ||
| Authors | ||
| Maissa Morsi* 1; Hala Abdelmigid2, 3; Nora Aljoudi4 | ||
| 1Department of Botany, Faculty of Women for Art, Science & Education, Ain Shams University, Egypt | ||
| 2Department of Biotechnology, Faculty of Science, Taif University, Taif, KSA | ||
| 3Department of Botany, Faculty of Science, Mansoura University, Mansura, Egypt | ||
| 4)Department of Biology, Faculty of Science, Taif University, Taif, KSA | ||
| Abstract | ||
| S ALT stress is a main factor limiting plant growth and productivity. Salicylic acid (SA) has been shown to alleviate the adverse effects of different environmental stresses on plants. To investigate the protective role of salicylic acid (SA) in alleviating salt stress on Rosmarinus officinalis L. (rosemary) plant, a pot experiment was conducted to measure the growth parameters such as plant height and branch number. An antioxidant defense system, represented by catalase (CAT), superoxide dismutase (SOD) and peroxidase (POX), was evaluated at both biochemical and transcriptional levels. NaCl was applied at 0, 25, 50 and 100mM and 0, 0.2 and 0.4mM of SA were used. The exposure of rosemary plant to salt conditions resulted in significant reduction of plant height, branches number and increases in the three measured antioxidant enzyme activities, compared to control. The foliar application of SA effectively increased growth rates and enhanced the activities of CAT, SOD and POX enzymes. The PCR (RT-PCR) analysis revealed that the relative transcript levels of SOD, CAT, and POX genes were changed and up-regulated compared to the control due to NaCl stress and SA treatments, reached the highest expression level by applying 25mM NaCl and 0 SA treatments. The results implied that SA regulated the transcript levels of the antioxidant genes, resulting in the increased contents of antioxidant enzymes and enhanced salt tolerance. | ||
| Keywords | ||
| salinity; rosemary; Antioxidant enzymes; Gene expression | ||
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