Quantitative determination of captopril, perindopril erbumine, moexipril hydrochloride, and ramipril in bulk and pharmaceutical preparations by high performance liquid chromatography | ||
| Records of Pharmaceutical and Biomedical Sciences | ||
| Article 4, Volume 1, Issue 1, March 2017, Pages 22-32 PDF (868.48 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/rpbs.2018.5917 | ||
| Authors | ||
| Mohamed Elgawish* 1; Samia M. Mostafa2; Abdalla A. Elshanawane3 | ||
| 1Medicinal Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, 41522, Egypt. | ||
| 2Medicinal Chemistry Department, Faculty of Pharmacy, University of Suez Canal, Ismailia 41522, Egypt | ||
| 3Medicinal Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt | ||
| Abstract | ||
| A high-performance liquid chromatographic method was developed for simultaneous determination of four ACE-inhibitors: captopril (CAP), perindopril erbumine (PER), moexipril hydrochloride (MOEX), and ramipril (RAM) in pharmaceutical formulations. The chromatographic separation was performed on Shim-pack cyanopropyl column with a mobile phase consisting of methanol-10mM ammonium acetate buffer (pH 6.0) in a ratio of (40: 60, v/v) at flow rate 1 ml min-1. the analysis was performed at ambient temperature using UV detector setting at 210 nm. All ACE-inhibitors were separated within seven min. The calibration curves were linear (r ≥ 0.9994) over a concentration range from 5 to 50 µg ml-1. the method was successfully applied to commercially available pharmaceutical preparations. The validity of the method was examined comparing the results obtained with official or published methods. | ||
| Keywords | ||
| Column liquid chromatography; ACE-Inhibitors; Pharmaceutical formulations | ||
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