EFFECT OF FISH OIL ON ALVEOLAR BONE OF RATS WITH INDUCED RHEUMATOID ARTHRITIS | ||
| Alexandria Dental Journal | ||
| Article 10, Volume 41, Issue 2, August 2016, Pages 176-181 PDF (1000.87 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/adjalexu.2016.59274 | ||
| Authors | ||
| Elkazzaz Nihal T.* ; AbdElSamad Salwa Y.* ; Kawana Khadiga Y.* ; Kora Azza S.* | ||
| Assistant lecturer of Oral Biology – Faculty of Dentistry – Alexandria University | ||
| Abstract | ||
| INTRODUCTION: Although rheumatoid arthritis (RA) is primarily a chronic inflammatory autoimmune disease which affects the synovial joints, it has profound effects on bone remodeling characterized by increase osteoclastic activity, and a negative balance of bone formation and resorption. A strong correlation has been reported between RA and periodontitis with subsequent alveolar bone resorption. As a potent anti-inflammatory mediator, fish oil rich in omega-3 polyunsaturated fatty acids (n-3 PUFA) has demonstrated beneficial actions in various inflammatory conditions including RA and cardiovascular disease. High intakes of n-3 PUFA provide symptomatic relief and reduce the doses of anti-inflammatory drugs needed to control the RA symptoms. OBJECTIVES: To evaluate the biological effect of fish oil on alveolar bone of rats with induced rheumatoid arthritis. MATERIALS AND METHODS: Twenty one male albino rats were used in this study. The rats were divided into 3 groups 7 rats each. Group I: control group, Group II: RA induced group and Group III: RA induced group + fish oil. Rheumatoid arthritis induction was done by single subcutaneous injection of Freund's complete adjuvant. Fish oil was orally gavaged daily till the end of the experiment. Laboratory investigations were performed twice, after RA induction and at the end of the experiment. Effect of fish oil on alveolar bone of rats was assessed histologically and ultrastructurely in the different groups. RESULTS: Remarkable improvement in the histology and the ultrastructure of the alveolar bone of rats in Group III was observed. Moreover, hematological values revealed significant decrease in the inflammatory condition of rats with induced RA after fish oil treatment. CONCLUSION: Rheumatoid arthritis is an important risk factor for alveolar bone loss. The treatment of the RA induced rats with fish oil not only prevented the alveolar bone resorption and stimulated new bone formation, but also reduced relatively the level of rheumatoid factor in the blood. | ||
| Keywords | ||
| alveolar bone; fish oil; Induced rheumatoid arthritis; Freund's complete adjuvant | ||
| References | ||
|
1. Bevaart L, Vervoordeldonk MJ, Tak PP. Evaluation of Therapeutic Targets in Animal Models of Arthritis. How Does It Relate to Rheumatoid Arthritis? Arthritis Rheum. 2010; 62:2192-205.
2. Grassi W, De Angelis R, Lamanna G, Cervini C. The clinical rheumatoid arthritis. Eur J Radiol. 1998; 27:18-24.
3. Block SR. Guidelines for the management of rheumatoid arthritis. Arthritis Rheum. 2002; 46:328-46.
4. Müller-Ladner U, Pap T, Gay RE, Neidhart M, Gay S. Mechanisms of disease: the molecular and cellular basis of joint destruction in rheumatoid arthritis. Nat Clin Pract Rheumatol. 2005; 1:102-10.
5. Haynes DR, Crotti TN, Loric M, Bain GI, Atkins GJ, Findlay DM. Osteoprotegrin and receptor activator of nuclear factor kappa B ligand (RANKL) regulate osteoclast formation by cells in the human rheumatoid arthritis joint. Rheumatology. 2001:40; 623-30.
6. Corrado A, Neve A, Maruotti N, Cantatore FP. Bone effects of biologic drugs in rheumatoid arthritis. Clin Dev Immunol. 2013; 2013:1-7.
7. Fit N, Chirilă F, Răpuntean S, Nadăş G, Preoteasa L, Cumpănaşu F. Haematological and biochemical investigations in rats with rheumatoid arthritis induced by Freund Complete Adjuvant and treated with bee venom. Veterinary Medicine. 2011; 68; 151-8.
8. Kässer UR, Gleissner C, Dehne F, Michel A, Willershausen-Zönnchen B, Bolten WW. Risk for periodontal disease in patients with longstanding rheumatoid arthritis. Arthritis Rheum. 1997; 40:2248-51.
9. Mercado F, Marshall R. Inter-relationships between rheumatoid arthritis and periodontal disease. Journal of Clinical Periodontology. 2003; 30:761-72.
10. Pischon N, Pischon T. Association among rheumatoid arthritis, oral hygiene, and periodontitis. Journal of Periodontology. 2008; 79:979-86.
11. Maroon JC, Bost JB. Nature's safest, most effective antiinflammatory: fish oil: the natural anti-inflammatory. Library of congress: E-Publishing; 2006:37.
12. Turchini GM, Wing-Keong NG, Tocher DR. Fish oil replacement and alternative lipid sources in aquaculture feeds. Tyalor and Francis group: E-Publishing; 2010:12-13.
13. Leeb BF, Sautner J, Andel I, Rintelen B. Intravenous application of omega-3 fatty acids in patients with active rheumatoid arthritis. Lipids. 2006;41:29–34
14. Simopoulos AP. The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp Biol Med. 2008; 233:674–88.
15. Firestein GS, Budd RC, Gabriel SE, Mcinnes IB, O'dell JR. Kelley's textbook of rheumatology. 9th ed. Elsevier Inc.; 2013:1049-52.
16. Kremer JM. N-3 fatty acids supplements in rheumatoid arthritis. Am J Clin Nutr. 2000; 71:349-51.
17. Kesavalu L, Vasudevan B, Raghu B, Browning E, Dawson D, Novak JM. Omega-3 fatty acid effect on alveolar bone loss in rats. J Dent Res. 2006:85:648-52.
18. Sun D, Krishnan A, Zaman K, Lawrence R, Bhattacharya A, Fernandes G. Dietary n-3 fatty acids decrease osteoclastogenesis and loss of bone mass in ovariectomized mice. J Bone Miner Res. 2003; 18:1206-16.
19. Hasturk H, Kantarci A, Ohira T, Arita M, Ebrahimi N, Chiang N. RvE1 protects from local inflammation and osteoclast-mediated bone destruction in periodontitis. FASEB J. 2006; 20:401-3.
20.Banji D, Pinnapureddy J, Otilia JF, Banji A, Kumar R. Evaluation of the concomitant use of methotrexate and curcumin on Freund's complete adjuvant-induced arthritis and hematological indices in rats. Narsi Reddy Indian J Pharmacol. 2011; 43: 546–50.
21. Vardar S1, Buduneli E, Türkoğlu O, Berdeli AH, Baylas H, Başkesen A, et al. Therapeutic versus prophylactic plus therapeutic administration of omega-3 fatty acid on endotoxin-induced periodontitis in rats. 2004; 75:1640-6.
22. Suresh P1, KavithaChN, Babu SM, Reddy VP, Latha AK. Effect of ethanol extract of Trigonella foenumgraecum (Fenugreek) seeds on Freund's adjuvant-induced arthritis in albino rats. 2012; 35:1314-21.
23.Bashkar SN. Orban oral histology and embryology. Torteno: Mosby; 1990:365-473.
24. Dunn CA, Jin Q, Taba M, Renny T. Franceschi, R. BMD gene delivery for alveolar bone engineering at dental implants defects. Molecular therapy. 2005; 11; 295-9.
25. Kirkpatrick LA, Feeney BC. A simple guide to IBM SPSS statistics for version 20.0. Student ed. Belmont, Calif.: Wadsworth, Cengage Learning; 2013.
26. Fit N, Chirila F, Rapuntean S, Nadas G. Haematological and Biochemical Investigations in Rats with Rheumatoid Arthritis Induced by Freund Complete Adjuvant and Treated with Bee Venom. Veterinary Medicine. 2011; 68:151-8.
27. Aizer J, Reed G, Onofrei A, Harisson MJ. Predictors of bone density testing in patients with rheumatoid arthritis. Rheumatol Int. 2009; 29:897–905.
28. Park JC, Su C, Jung IH, Choi SH. Mechanism of alveolar bone loss in a collagen-induced arthritis model in mice. 2011; 38:122-30.
29.Bernstein CN, Blanchard JF, Leslie W, Wajda A. The incidence of fracture among patients with inflammatory bowel disease: a population-based cohort study. Annals of Internal Medicine. 2000; 133:95-100.
30.Bultink IEM, Lems WF, Kostense PJ, Dijkmans BAC, Voskuyl AE. Prevalence of and risk factors for low bone mineral density and vertebral fractures in patients with systemic lupus erythematosus. Arthritis and Rheumatism. 2005; 52: 2044–50.
31. Sambrook PN. The skeleton in rheumatoid arthritis: common mechanisms for bone erosion and osteoporosis. J Rheumatol. 2000; 27:2541-2.
32.Bahadori B, Uitz E, Thonhofer R. Omega-3-fatty acid infusions as adjuvant therapy in rheumatoid arthritis. J Parenter Enteral Nutr. 2010; 34:151-5.
33.Ruggiero C, Lattanzio F, Lauretani F, Gasperini B, AndresLacueva C, Cherubini A. Omega-3 polyunsaturated fatty acids and immune-mediated diseases: inflammatory bowel disease and rheumatoid arthritis. 2009; 15:4135-48.
34. Alam SQ, Kokkinos PP, Alam BS. Fatty acid composition and arachidonic acid concentrations in alveolar bone of rats fed diets with different lipids. Calcif Tissue Int. 1993; 35- 52.
35. Serhan CN, Gotlinger K, Hong S, Arita M. Resolvins, docosatrienes, and neuroprotectins, novel omega-3-derived mediators, and their aspirin-triggered endogenous epimers: an overview of their protective roles in catabasis. Prostaglandins Other Lipid Mediat. 2004; 73:155–72.
36. Herrera BS, Ohira T, Gao L, Omori K, Yang R. An endogenous regulator of inflammation, resolvin E1, modulates osteoclast differentiation and bone resorption. Br J Pharmacol. 2008; 155:1214–23.
37. Poulsen RC, Gotlinger KH, Serhan CN, Kruger MC. Identification of inflammatory and proresolving lipid mediators in bone marrow and their lipidomic profiles with ovariectomy and omega-3 intake. Am J Hematol. 2008; 83:437–45.
38.James MJ, Gibson RA, Cleland LG. Dietary polyunsaturated fatty acids and inflammatory mediator production. Am J Clin Nutr. 2000; 71:343–8.
39. Poulsen RC, Moughan PJ, Kruger MC. Longchainpolyunsaturated fatty acids and the regulation of bone metabolism. Exp Biol Med. 2007; 232:1275–88. | ||
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