RNA INTERFERNCE (RNAI) FOR SOME GENES FROM BABESIA BOVIS | ||
| Research Journal of Applied Biotechnology | ||
| Article 9, Volume 2, Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology, November 2016, Pages 81-92 PDF (705.53 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/rjab.2016.59638 | ||
| Authors | ||
| Mahmoud AbouLaila* 1; Naoaki Yokoyama2; Ikuo Igarashi2 | ||
| 1Department of Parasitology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Minoufiya, Egypt | ||
| 2National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan | ||
| Abstract | ||
| Babesia parasites are responsible for enormous economic losses to the livestock industry worldwide. There is no vaccine available to protect from the infection. RNA interference (RNAi) has emerged as a simple and incisive technique to study gene functions in a variety of organisms. In this study, we evaluated the effects of RNAi by double-stranded RNA (dsRNA) of the DNA gyrase subunits A and B, the DNA-directed RNA polymerase beta subunit (rpo B), and thiostrepton interaction site (ribosomal L11 protein) genes on the in vitro growth of Babesia bovis. The inhibitory effects produced by dsRNAs of DNA gyrase subunit A, DNA gyrase subunit B, thiostrepton interaction site (L11) and the DNA-directed RNA polymerase beta subunit (rpo B1and B2) were compared with the effect of drugs target these genes. RNAi directed towards these genes resulted in inhibition of in vitro growth of the parasite. The RT-PCR showed that parasites treated with dsRNAs of DNA gyrase subunit A, DNA gyrase subunit B, ribosomal L11, and the DNA-directed RNA polymerase beta subunit (rpo B1 and B2) have blocked expression of corresponding endogenous mRNAs of DNA gyrase subunit A, DNA gyrase B, ribosomal L11, and rpo B genes. These results demonstrate that the RNAi technique disrupted gene function in B. bovis and the mechanism of action required further studies to be elucidated. | ||
| Keywords | ||
| Apicoplast; RNAi; in Vitro; Babesia bovis | ||
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