ELFARASH, A., SOLORIO-SÁNCHEZ, F., ROJAS-HERRERA, R., TOLEDO-LOPEZ, V., EL-DERWY, Y. (2016). PCR DETECTION OF PATHOGENIC BACTERIA IN MILK AND SOFT CHEESE UNDER DIFFERENT COW’S PRODUCTION SYSTEMS IN MEXICO. test, 2(Special issue (2) for the first International Conference of Genetic Engineering and Biotechnology), 107-116. doi: 10.21608/rjab.2016.59904
Ameer E. ELFARASH; Francisco J. SOLORIO-SÁNCHEZ; Rafael A. ROJAS-HERRERA; Victor M. TOLEDO-LOPEZ; Yaser M. A. EL-DERWY. "PCR DETECTION OF PATHOGENIC BACTERIA IN MILK AND SOFT CHEESE UNDER DIFFERENT COW’S PRODUCTION SYSTEMS IN MEXICO". test, 2, Special issue (2) for the first International Conference of Genetic Engineering and Biotechnology, 2016, 107-116. doi: 10.21608/rjab.2016.59904
ELFARASH, A., SOLORIO-SÁNCHEZ, F., ROJAS-HERRERA, R., TOLEDO-LOPEZ, V., EL-DERWY, Y. (2016). 'PCR DETECTION OF PATHOGENIC BACTERIA IN MILK AND SOFT CHEESE UNDER DIFFERENT COW’S PRODUCTION SYSTEMS IN MEXICO', test, 2(Special issue (2) for the first International Conference of Genetic Engineering and Biotechnology), pp. 107-116. doi: 10.21608/rjab.2016.59904
ELFARASH, A., SOLORIO-SÁNCHEZ, F., ROJAS-HERRERA, R., TOLEDO-LOPEZ, V., EL-DERWY, Y. PCR DETECTION OF PATHOGENIC BACTERIA IN MILK AND SOFT CHEESE UNDER DIFFERENT COW’S PRODUCTION SYSTEMS IN MEXICO. test, 2016; 2(Special issue (2) for the first International Conference of Genetic Engineering and Biotechnology): 107-116. doi: 10.21608/rjab.2016.59904

PCR DETECTION OF PATHOGENIC BACTERIA IN MILK AND SOFT CHEESE UNDER DIFFERENT COW’S PRODUCTION SYSTEMS IN MEXICO

Research Journal of Applied Biotechnology
Article 10, Volume 2, Special issue (2) for the first International Conference of Genetic Engineering and Biotechnology, June 2016, Pages 107-116    PDF (515 K)
Document Type: Original Article
DOI: 10.21608/rjab.2016.59904
Authors
Ameer E. ELFARASH* 1; Francisco J. SOLORIO-SÁNCHEZ2; Rafael A. ROJAS-HERRERA3; Victor M. TOLEDO-LOPEZ4; Yaser M. A. EL-DERWY5
1Department of Genetics, Faculty of Agriculture, Assiut University, Assiut (71526), Egypt.
2Faculty of Veterinary Medicine and Animal Science (CCBA), Autonomous University of Yucatan (UADY), Merida, Yucatan (97100), Mexico.
3Faculty of Chemical Engineering, Autonomous University of Yucatan (UADY), Merida, Yucatan (97203), Mexico.
4Merida Institute of Technology, Merida, Yucatan (97118.), Mexico.
5Faculty of Veterinary Medicine and Animal Science (CCBA), Autonomous University of Yucatan (UADY), Merida, Yucatan (97100), Mexico. Department of Dairy science, Faculty of Agriculture, Assiut University, Assiut (71526), Egypt.
Abstract
Thirty six samples of raw milk were collected from three different farms located in Merida, Yucatan, Mexico and seventy two samples of soft cheese were manufactured from thirty six samples of raw milk and thirty six samples of pasteurized milk from the previous collected raw milk during May 2013 to April 2014. The first and second farm located at Faculty of Veterinary Medicine and Animal Science, Autonomous University of Yucatan (FMVZ/UADY) used two different production regimes; intensive silvopastoral system (ISS) and traditional system (TS). The third farm in Kampepen under (ISS). The collected raw milk samples were divided into three portions, one portion of raw milk and two portions for soft cheese manufacture from (raw and pasteurized milk) for PCR screening. PCR screening was performed for one hundred and eight samples to detect four types of the most dangerous foodborne pathogens (Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, Staphylococcus aureus and Listeria monocytogenes). PCR results showed that almost samples were negative for all tested pathogens except six samples (5.56%); 3 samples of raw milk and other 3 samples of soft cheese manufactured from this raw milk under traditional system which were positive for Staphylococcus aureus.
Keywords
PCR; Pathogenic bacteria; Raw milk; Soft cheese; Production systems
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