Identity of Salmonella Enterica Serovar Enteritidis, Typhimurium and Kentucky Local Strains Used In Preparation of Polyvalent Inactivated Salmonella Vaccine in Chickens in Egypt. | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Article 7, Volume 23, Issue 1, June 2018, Pages 85-93 PDF (832.78 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2018.60769 | ||
| Authors | ||
| Ahmed Khafagy1; Salama Sselim2; Rasha Ibrahim* 1 | ||
| 1Dept. of Bact., Immun. and Mycology, Faculty of Vet. Med., Suez Canal University, Egypt. | ||
| 2Central Laboratory for Evaluation of Veterinary biologics (CLEVB), Abbasia, Cairo, Egypt. | ||
| Abstract | ||
| Salmonellosis is one of the most important bacterial diseases affecting poultry. Its importance is derived from the loss in productivity in affected birds and the hazard it causes for public health. For preparation of polyvalent inactivated Salmonella vaccine, we tested the local virulent Salmonella Typhimurium, Salmonella Enteritidis and Salmonella Kentucky strains phenotypically by the microscope, biochemically by API test and genotypically by Polymerase Chain Reaction (PCR) using a specific primer for fliC gene of Salmonella Typhimurium and a specific primer for sefA gene of Salmonella Enteritidis and a specific primer for invA gene of Salmonella Kentucky. In Conclusion: Salmonellae are gram negative rods. They are aerobic or facultative anaerobic organisms that ferment sugars, producing gas and H2S and that have a complex antigenic structure (somatic "O", flagellar "H", and capsular "K" antigens). Multiplex PCR reveals that Salmonella Kentucky, Salmonella Typhimurium and Salmonella Enteritidis were positive for invA gene with a product of 284bp, fliC gene with a product of 620bp and sefA gene with a product of 488bp respectively. | ||
| Keywords | ||
| Salmonella enterica serovar enteritidis; typhimurium; Kentucky; Polyvalent Inactivated Salmonella Vaccine; chickens | ||
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