Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth | ||
| Journal of Applied Veterinary Sciences | ||
| Article 10, Volume 1, Issue 1, October 2016, Pages 69-72 PDF (418.32 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/javs.2016.62123 | ||
| Authors | ||
| A. T. El-Dakhly* 1; M. M. Youssef1; Abeer A. Tammam1; Namaa A. Moedham2 | ||
| 1Rinder Pest Vaccine Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt | ||
| 2Pox Vaccines Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt | ||
| Abstract | ||
| This work presents cultural characterization of Peste Des Petites Ruminants (PPR) virus in VERO-culture. The VERO cells are currently considered as an acceptable cell substrate to produce a wide range of viruses. This study evaluates the best time for inoculation of PPR virus on VERO cell cultures; the study proved that the optimum time was 24 hours after subculture of VERO cell line using MOI 2: 1. It was also found that the best time of harvstation of virus fluid of PPR was 9th day post inoculation to reach the best titre 6 log10 TCID50 /ml. | ||
| Keywords | ||
| VERO cell; Peste Des Petits Ruminants; cell growth | ||
| References | ||
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