Detection of Virulence Genes of Staphylococcus Aureus Isolated from Chicken Using Polymerase Chain Reaction. | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Article 2, Volume 22, Issue 1, June 2017, Pages 19-29 PDF (749.1 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2017.62375 | ||
| Authors | ||
| Hamza Eid1; Amany Shalaby2; Mera Shtewy* 3 | ||
| 1Department of Microbiology, Faculty of Veterinary Medicine, Suez Canal University. | ||
| 2Department of Food Hygiene, Animal Health Research Institute, Port Said Branch | ||
| 3Veterinarian | ||
| Abstract | ||
| A total of 300 chicken samples (100 samples for breast muscles, 100 samples for thigh muscles and 100 samples for joints) were collected randomly from different markets in Port-said city during the period of work from July 2015 till August 2016. Overall percentage of coagulase positive S. aureus which isolated from chicken samples were 37.2% (49/132) while coagulase positive S. aureus of breast muscles, thigh muscles and joints were 15.1%, 14.5% and 7.6% respectively. PCR revealed that 15 tested isolates were S. aureus using 16SrRNA at 791 bp. The study conducted to detect virulence genes, coa 15 isolates were positive at 570 bp. (100%), clfA15 isolates were positive at 638 bp. (100%), See gene is the highest SE, 5 isolates were positive at 209 bp. (33.33%), followed by Seb gene 1isolate were positive at164 bp. (6.67%). None of 15 isolates were positive for Sea, Sec and Sed. | ||
| Keywords | ||
| Staphylococcus aureus; Detection of Virulence Genes; chicken; polymerase chain reaction | ||
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