Khafagy, A., El Gamal, R., F., H., A., S. (2017). Multiplex PCR of Some Antibiotic Resistance Genes of Methicillin Resistant Staphylococcus Aureus (MRSA) Isolated from Infected Cat Fish (Clarias garipeneaus).. test, 22(1), 31-42. doi: 10.21608/scvmj.2017.62376
Ahmed Khafagy; R. El Gamal; Hala F.; Samaa A.. "Multiplex PCR of Some Antibiotic Resistance Genes of Methicillin Resistant Staphylococcus Aureus (MRSA) Isolated from Infected Cat Fish (Clarias garipeneaus).". test, 22, 1, 2017, 31-42. doi: 10.21608/scvmj.2017.62376
Khafagy, A., El Gamal, R., F., H., A., S. (2017). 'Multiplex PCR of Some Antibiotic Resistance Genes of Methicillin Resistant Staphylococcus Aureus (MRSA) Isolated from Infected Cat Fish (Clarias garipeneaus).', test, 22(1), pp. 31-42. doi: 10.21608/scvmj.2017.62376
Khafagy, A., El Gamal, R., F., H., A., S. Multiplex PCR of Some Antibiotic Resistance Genes of Methicillin Resistant Staphylococcus Aureus (MRSA) Isolated from Infected Cat Fish (Clarias garipeneaus).. test, 2017; 22(1): 31-42. doi: 10.21608/scvmj.2017.62376

Multiplex PCR of Some Antibiotic Resistance Genes of Methicillin Resistant Staphylococcus Aureus (MRSA) Isolated from Infected Cat Fish (Clarias garipeneaus).

Suez Canal Veterinary Medical Journal. SCVMJ
Article 3, Volume 22, Issue 1, June 2017, Pages 31-42    PDF (583.95 K)
Document Type: Original Article
DOI: 10.21608/scvmj.2017.62376
Authors
Ahmed Khafagy1; R. El Gamal2; Hala F.2; Samaa A.* 3
1Dept. of Microbiology Faculty of Vet. Medicine, Suez Canal University, Ismailia, Egypt.
2Central laboratory for Aquaculture Research, Agriculture Research Center, Egypt
3Department of Microbiology, Faculty of Vet. Medicine, Suez Canal University, Ismailia, Egypt.
Abstract
 This study was designed to detect some antibiotic resistance genes of S.aureus by using PCR. A total of 150 Cat fish (Clarias garipeneaus) with a body weight ranged from200 – 250g.  suffered from ulcerated skin and erosion. S. aureus were isolated in percent of 100% from infected fish by using Baird parker as selective media. By using multiplex PCR for investigation some antibiotic resistant genes, as erm A gen,  aac (6)-aph (2) genes , van A gen and  mec A gene. All isolated S. aureus were sensitive to Amikacin, Amoxicillin, Ampicillin and Ciprofloxacin while resistant to Gentamicin, Methicillin and Neomycin.
Keywords
PCR; Antibiotic resistance genes; Staphylococcus aureus; Cat Fish
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