Eissa, I., El Lamie, M., Ismail, M., Abd Elrehim, A. (2016). Studies on Edwardsiellosis in Some Marine Fishes Using Molecular Diagnosis at Suez bay. test, 21(2), 57-66. doi: 10.21608/scvmj.2016.62652
Ismail Eissa; Maather El Lamie; Mona Ismail; A. Abd Elrehim. "Studies on Edwardsiellosis in Some Marine Fishes Using Molecular Diagnosis at Suez bay". test, 21, 2, 2016, 57-66. doi: 10.21608/scvmj.2016.62652
Eissa, I., El Lamie, M., Ismail, M., Abd Elrehim, A. (2016). 'Studies on Edwardsiellosis in Some Marine Fishes Using Molecular Diagnosis at Suez bay', test, 21(2), pp. 57-66. doi: 10.21608/scvmj.2016.62652
Eissa, I., El Lamie, M., Ismail, M., Abd Elrehim, A. Studies on Edwardsiellosis in Some Marine Fishes Using Molecular Diagnosis at Suez bay. test, 2016; 21(2): 57-66. doi: 10.21608/scvmj.2016.62652

Studies on Edwardsiellosis in Some Marine Fishes Using Molecular Diagnosis at Suez bay

Suez Canal Veterinary Medical Journal. SCVMJ
Article 6, Volume 21, Issue 2, December 2016, Pages 57-66    PDF (1.11 M)
Document Type: Original Article
DOI: 10.21608/scvmj.2016.62652
Authors
Ismail Eissa1; Maather El Lamie1; Mona Ismail1; A. Abd Elrehim* 2
1Dept. of Fish Diseases and Management, Faculty of Vet. Medicine, Suez Canal Univ.
2Royal International Inspection Lab. (RIIL), Ain-Elsokhna, Suez governorate
Abstract
A total of 240 marine fishes Mugil cephalus, Rastrelliger kanagurta and Nemipterus japonicas were randomly collected from Attaka fishing port, Suez-bay, Suez Governorate (60 in each winter and fall and 120 in spring).The infected fishes showed hemorrhages all over the body surface including gills cover and fins, scales detachment accompanied by slight swelling of abdomen. Isolation was done from gills, liver, kidneys and intestines using Tryptone Soya Agar medium and X.L.D medium. Morphological and biochemical identification of bacterial isolates revealed three different isolates of Edwardsiella tarda (A, B and C). Their diagnosis was confirmed using Microbact 24E and 16S RNA gene for PCR. Their virulence was differentiated by H2S, Indole, Catalase test and haemolysis on blood agar (α, β and γ). The total prevalence of E. tarda isolates was (9.6%) among all examined marine fishes while seasonal prevalence was (15%) in spring, (3.3%) in winter and (5%) in fall.
Keywords
Edwardsiellosis; E. tarda; Mugil cephalus; Rastrelliger kanagurta; Nemipterus japonicas; PCR
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