Reduction Of Microbial Contamination Of Whole Broiler Chicken Carcasses During Processing | ||
| Journal of Applied Veterinary Sciences | ||
| Article 2, Volume 4, Issue 1, April 2019, Pages 5-12 PDF (354.78 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/javs.2019.62670 | ||
| Authors | ||
| F.A. Khalafalla1; N.S. Abdel Atty1; Soad A. Nasef2; Adel S. Hanafy* 3 | ||
| 1Faculty of veterinary medicine, Beni suef university, Egypt | ||
| 2Animal Health Research Institute, Dokki, Giza | ||
| 3Animal Health Research Institute, Dokki, Giza, Egypt | ||
| Abstract | ||
| Contamination of broiler carcasses during processing with several microorganisms as salmonella, campylobacter, E. coli and staphylococcus aureus is frequently occurring. Scalding, defeathering, chilling are critical points at which cross contamination may occur during processing. Recently several interventions for carcass decontamination have been employed in order to reduce the levels of microbial hazards on poultry carcasses during processing; among which chlorine and organic acids are the most common. This study was carried out in a traditional poultry abattoir in Fayoum Governorate in order to improve the microbial quality of broiler carcasses. During 10 replicate a total of 160 carcasses were collected at 4 sampling points in the processing line (scalding, defeathering, final wash and chilling). Bacterial counts recovered from broiler carcasses rinse were lowered by 1.3, 1.3, 0.3 and 0.5 Log10 cfu\ ml after treatment of scalding water with 100 ppm Calcium hypochlorite in scalding. Adding peracetic acid (PAA) 50 ppm + 0.5 % acetic acid to the defeathering machine spraying system during defeathering lowered the count of coliforms and faecal coliforms significantly (P ≤ 0.05) by 1.5 and 1.6 Log10 cfu\ ml of carcass rinse, while it wasn't significant for E. coli and staphylococcus counts (1.2 and 0.95 Log10 cfu\ ml), respectively. Furthermore, significant differences in the reduction of all bacterial counts were observed in the washing stage (P ≤ 0.05) after treatment of broiler carcasses with a mixture of lactic acid 1 % + acetic acid 1 % in the final washing step. At chilling stage Na hypochlorite 50 ppm reduced the bacterial counts by 2, 2.2, 1.3 and 0.8 log10 cfu\ ml of carcass rinse for coliforms, faecal coliforms, Escherichia coli and staphylococcus aureus, respectively. E. coli, salmonellae, staphylococcus aureus and campylobacter spp. were reduced by different percentages. The used interventions effectively or significantly reduced microbial populations on broiler chicken carcasses during processing. | ||
| Keywords | ||
| poultry processing; Decontamination; Chlorine; Peracetic acid; lactic acid; acetic acid | ||
| References | ||
|
ABU-RUWAIDA, A.S., SAWAYA, W.N., DASHTI, B.H., MURAD, M. AND AL-OTHMAN, H.A. 1994. Microbiological quality of broilers during processing in a modern commercial slaughterhouse in Kuwait. J. food prot., 57: 887-892.
ALLEN, V.M., BULL, S.A., CORRY, J.E., DOMINGUE, G., JØRGENSEN, F., FROST, J.A., WHYTE, R., GONZALEZ, A., ELVISS, N. AND HUMPHREY, T.J. 2007. Campylobacter spp. contamination of chicken carcasses during processing in relation to flock colonisation. Int. J. Food Microbiol, 113: 54-56.
BAILEY, J. S., THOMSON, J. E., COX, N. A. AND SHACKELFORD, A. D. (1986): Chlorine spray washing to reduce bacterial contamination of poultry processing equipment. Poult. Sci., 65: 1120–1123.
BASHOR, M. P., CURTIS, P. A., KEENER., K. M., SHELDON, B. W., KATHARIOU, S. AND OSBORNE, J. A. 2004. Effects of Carcass Washers on Campylobacter Contamination in Large Broiler Processing Plants. Poult. Sci., 83: 1232- 1239. BAUERMEISTER, L.J., BOWERS, J.W.J., TOWNSEND, J.C. AND MCKEE, S.R. 2008. Validating the efficacy of peracetic acid mixture as an antimicrobial in poultry chillers. J. Food Prot., 7: 119–122.
BERRANG, M. E. AND BAILEY, J. S. 2009. On-line brush and spray washers to lower numbers of Campylobacter and Escherichia coli and presence of Salmonella on broiler carcasses during processing. J. Appl. Poult. Res., 18: 74–78.
BERRANG, M. E., BUHR, R. J., CASON, J. A. AND DICKENS, J. A. 2001. Broiler Carcass Contamination with Campylobacter from Feces during Defeathering. J. Food Prot., 64: 2063–2066.
BERRANG, M. E., MEINERSMANN, R. J., COX, N. A. AND FEDORKA-CRAY, P. J.2011. Application of chlorine dioxide to lessen bacterial contamination during broiler defeathering. J. Appl. Poult. Res., 20 : 33–39.
BERRANG, M.E., BUHR, R.J AND CASON, J.A. 2000. Campylobacter recovery from external and internal organs of commercial broiler carcass prior to scalding. Poult. Sci., 79: 286–290.
BYRD, J.A. AND MCKEE, S.R. 2005. Improving slaughter and processing technologies. In: Mead, G.C. (Ed.), Food Safety Control in the Poultry Industry. CRC Press LLC, Boca Raton, FL, pp. 310–327.
CASON, J. A., BAILEY, J. S., STERN, N. J., WHITTEMORE, A. D. AND COX, N. A. 1997. Relationship between aerobic bacteria, salmonellae, and Campylobacter on broiler carcasses. Poult. Sci. 76: 1037–1041.
DICKENS, J. A. AND WHITTEMORE, A. D. 1997. Effects of Acetic Acid and Hydrogen Peroxide Application During Defeathering on the Microbiological Quality of Broiler Carcasses Prior to Evisceration. Poult. Sci., 76: 657–660.
DICKENS, J. A. AND WHITTEMORE, A. D. 1994. The effect of acetic acid and air injection on appearance, moisture pick-up, microbiological quality, and Salmonella incidence on processed poultry carcasses. Poult. Sci., 73: 582–586.
DICKENS, J.A. AND WHITTEMORE, A.D. 1995. The effects of extended chilling times with acetic acid on the temperature and microbiological quality of processed poultry carcasses. Poult. Sci., 74: 1044-1048. DICKSON, J. S. AND ANDERSON, M. E. 1992. Microbiological decontamination of food animal carcasses by washing and sanitizing systems: a review. J. Food Prot., 55: 133-140.
DINCER, A. H., BAYSAL, T. 2004. Decontamination techniques of pathogen bacteria in meat and poultry. Crit. Rev. Microbiol., 30: 197- 204. FABRIZIO, K. A., SHARMA, R. R., DEMIRCI, A. AND CUTTER, C. N. 2002. Comparison of electrolyzed oxidizing water with various antimicrobial interventions to reduce Salmonella species on poultry. Poult. Sci., 81: 1598–1605.
FDA (2002): BAM (Bacteriological Analytical Manual) Chapter4: Enumeration of Escherichia coli and the Coliform Bacteria.
GEORNARAS, I., DE JESUS, A. E., VAN ZYL, E. AND VAN HOLY, A.1997. Bacterial populations of different sample types from carcasses in the dirty area of a South African poultry abattoir. J. Food Prot., 60: 551–554.
GÖKSOY, E. O., KIRKAN, S. AND KOK, F. 2004. Microbiological quality of broiler carcasses during processing in two slaughterhouses in Turkey. Poult. Sci., 83: 1427–1432.
GRAPHPAD SOFTWARE, INSTAT GUIDE TO CHOOSING AND INTERPRETING STATISTICAL TESTS 1998. GraphPad Software, San Diego, CA, USA, http://www.graphpad.com.
INTERNATIONAL ORGANIZATION FOR STANDARDIZATION, ISO 10272 2006. Microbiology of Food and Animal Feeding Stuffs - Horizontal method for enumeration of campylobacter spp. part 1 detection method. Geneva, Switzerland. INTERNATIONAL ORGANIZATION FOR STANDARDIZATION, ISO 6579 .2002. Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the detection of Salmonella spp. ISO, Geneva, Switzerland. INTERNATIONAL ORGANIZATION FOR STANDARDIZATION, ISO 6888 .1999. Microbiology of Food and Animal Feeding Stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) – part 1: technique using Baird-Parker agar medium. Geneva, Switzerland. Izat, A. L., Colberg, M., Adams, M. H., Reiber, M. A. and. WALDROUP P. W. 1989. Production and processing studies to reduce the incidence of salmonellae on commercial broilers. J. Food Prot. 52:670-673.
Izat, A. L., Coldberg, M., Thomas, R. A., Adams, M. H. and DRIGGERS, C. D. 1990. Effects of lactic acid in processing waters on the incidence of Salmonella on broilers. J. Food Qual., 13:295–306.
KILLINGER, K.M., KANNAN, A., BARY, A.I. AND COGGER, C.G. 2010. Validation of a 2 Percent Lactic Acid Antimicrobial Rinse for Mobile Poultry Slaughter Operations. J. Food Prot., 73: 2079-2083. LILLARD, H. S. 1980. Effect on broiler carcasses and water of treating chiller water with chlorine or chlorine dioxide. Poult. Sci., 59: 1761–1766.
LILLARD, H. S., BLANKENSHIP, L. C., DICKSON, J. A., CRAREN, S. E. AND SHACKELFORD, A. D. 1987. Effect of acetic acid on the microbiological quality of scalded picked and unpicked broiler carcasses. J. Food Prot., 50: 112–114.
MEAD G. C., HUDSON, W.R. AND HINTON, M.H. 1995. Effect of changes in processing to improve hygiene control on contamination of poultry carcasses with campylobacter. Epidemiol. Infect., 115: 495-500. NAGEL, G. M., BAUERMEISTER, L. J., BRATCHER, C. L., SINGH, M. AND MCKEE., S. R. 2013. Salmonella and Campylobacter reduction and quality characteristics of poultry carcasses treated with various antimicrobials in a post-chill immersion tank. Int. J. Food Microbiol. 165: 281–286.
NIDAULLAH, H., ABIRAMI, N., SHAMILA-SYUHADA, A. K., CHUAH, L. O., NURUL, H., TAN, T. P., ABIDIN, F.W. Z. AND RUSUL, G. 2017. Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia. Vet. World., 10: 286 -292.
NORTHCUTT, J. K., BERRANG, M. E., SMITH, D. P. AND JONES, D. R. 2003.: Effect of commercial bird washers on broiler carcass microbiological characteristics. J. Appl. Poult. Res., 12: 435– 438.
NORTHCUTT, J. K., SMITH, D. P., MUSGROVE, M. T., INGRAM, K. D. AND HINTON, JR. A. 2005. Microbiological impact of spray washing broiler carcasses using different chlorine concentrations and water temperatures. Poult. Sci., 84: 1648–1652.
OKOLOCHA, E. C. AND ELLERBROEK, L. 2005. The influence of acid and alkaline treatments on pathogens and the shelf life of poultry meat. Food Control., 16: 217–225.
RASSCHAERT, G., PIESSENS, V., SCHELDEMAN, P., LELEU, S., STALS, A., HERMAN, L., HEYNDRICKX, M. AND MESSENS, W. 2013. Efficacy of electrolyzed oxidizing water and lactic acid on the reduction of Campylobacter on naturally contaminated broiler carcasses during processing. Poult Sci., 92: 1077-1084. ROSENQUIST, H., SOMMER, H.M., NIELSEN, N.L. AND CHRISTENSEN, B.B. 2006. The effect of slaughter operations on the contamination of chicken carcasses with thermotolerant Campylobacter. Int. J. Food. Microbiol., 108: 226-232.
SAKHARE, P. Z., SACHINDRA, N. M., YASHODA, K. P. AND RAO, D. N. 1999. Efficacy of intermittent decontamination treatments during processing in reducing the microbial load on broiler chicken carcass. Food Control., 10: 189–194.
SCALLAN, E., HOEKSTRA, R. M., ANGULO, F. J., TAUXE, R. V., WIDDOWSON, M. A. AND ROY, S. L. 2011. Foodborne illness acquired in the United States-major pathogens. Emerg. Infect. Dis., 17: 7-15. STOPFORTH, J. D., O’CONNOR, R., LOPES, M., KOTTAPALLI, B., HILL, W. E. AND SAMADPOUR, M. 2007. Validation of individual and multiple-sequential interventions for reduction of microbial populations during processing of poultry carcasses and parts. J. Food Prot. 70: 1393–1401.
TSAI, L. S., HIGBY, R. AND SCHADE, J. 1995. Disinfection of poultry chiller water with chlorine dioxide: consumption and byproduct formation. J. Agric. Food Chem. 43: 2768–2773.
XIONG, H., LI, Y., SLAVIK, M. F. AND WALKER, J. T. 1998. Spraying chicken skin with selected chemicals to reduce attached Salmonella typhimurium. J. Food Prot., 61: 272–275.
YANG, H., LI, Y. AND JOHNSON, M. G. 2001. Survival and death of Salmonella typhimurium and Campylobacter jejuni in processing water and on chicken skin during poultry scalding and chilling. J. Food Prot., 64: 770-776.
YANG, Z., LI, Y. AND SLAVIK, M. 1998. Use of antimicrobial spray applied with an inside-outside bird washer to reduce bacterial contamination on prechilled chicken carcasses. J. Food Prot. 61, 829–832. | ||
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