Comparative Studies on Infectious Bursal Disease Virus Propagated in Chicken Eggs and Vero Cell Culture | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Article 11, Volume 21, Issue 1, June 2016, Pages 109-120 PDF (687.08 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2016.62766 | ||
| Authors | ||
| Mohamed Khodeir1; Mokhtar El Tarabeily2; Heba El Masry* 3; Shaira Abdelwahab4; Mohamed Fawzy4 | ||
| 1Veterinary Serum and Vaccine Research Institute, Abassia, Cairo | ||
| 2Virology Department, Faculty of Veterinary Medicine, Suez Canal University | ||
| 3Free Veterinarian | ||
| 4Virology Department,Faculty of Veterinary Medicine Suez Canal University | ||
| Abstract | ||
| Ten bursal samples were pooled from the IBD infected birds in Damietta Governorate showing haemorrhagic and enlarged bursae. Bursal homogenates were used for ten-day-old embryonated specific pathogen free embryonated tests included VN and FAT tests revealed the incidence of IBDV antigen. Propagation of the obtained isolate in ECE and Vero cells for 10 passages revealed gradual increase in the virus titer up to 8 log10EID50/ml and 7.5TCID50/ml respectively. Original, egg adapted, cell culture adapted and bursavac samples were used for viral RNA extraction by using reagent---- (Trizol). RT-PCR was performed for samples using V1 and V2 pair of primers of hypervariable region of VP2 gene to confirm the presence of IBDV in cell culture. The results of Viral RNA extracted from all samples is generation of a targeted amplicon of 472 bp confirming that there were no differences between any of them. | ||
| Keywords | ||
| Bursal Disease Virus; Chicken eggs; Vero Cell Culture | ||
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