Biochemical and phytochemical studies on Balanities aegyptiaca fruits | ||
| Biochemistry Letters | ||
| Article 1, Volume 11, Issue 1, 2015, Pages 13-26 PDF (787.83 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/blj.2015.63388 | ||
| Authors | ||
| Faten Zahran Mohamed1; Abdelgawad Ali Fahmi2; Amr Saad3; Fathy Mohamed Mehaya4; Esraa ahmed5 | ||
| 1Chemistry department, Biochemistry Division, Faculty of science, Zagazig university, Cairo, Egypt | ||
| 2Chemistry department, Faculty of science, Cairo university, Cairo, Egypt | ||
| 3Biochemistry Department, Faculty of Science, Cairo University, Egypt | ||
| 4food technology department, National Research Center, Egypt | ||
| 5environmental researches department, National Research Center, Egypt | ||
| Abstract | ||
| Objectives: Phytochemical screeninig of Balanities aegyptiaca (B.a.) extract and invitro determination of some biochemical effects. Methods: preparation of defatted Balanities aegyptiaca total extract, it's characterization was done throughout Firstly, quantitative and qualitative determination of active phenolic components by HPLC and spectrophotometer analysis. Secondly, identification of different function groups by IR analysis and surface elements by electronic microscope, then estimation for saponins content by spectrophotometer, After that estimation of total flavonoids, carbohydrates and proteins. Finally, determination of B. a. extract antioxidant activity by using DPPH, anti-inflammatory activity and antitumor activity using (MCF-7) human breast adenocarcinoma cell line. Results: This study revealed that total extract of Balanities fruits showed high levels of antioxidants as each 1 gram contained 10 mg of ascorbic acid and anti-inflammatory activities as it inhibited NO production by 60% compared to positive control group. Also, it revealed high antiproliferation activity IC50=21.2 ug/ml correlated with its phytochemical screening which showed the presence of high levels of phenolics, flavnoids, minerals (Zn, Mg, Ca, K, Na, Fe, Cl, Al, Si, O and Carbon) and Saponins. Conclusion: Balanities aegyptiaca extract plays an important role in improving oxidative stress resulted in high antiproliferation activity against (MCF-7) and posses a strong anti-inflammatory agent, Furthermore purification and isolation of active compounds will help in recommendation of new antinflammatory and antitumor herbal treatments. | ||
| Keywords | ||
| Balanities aegyptiaca; Phytochemical screening; invitro anti-inflammatory; antitumor activity | ||
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