Tolba, Y., Omar, S., Nagy, D. (2019). A BIOCHEMICAL STUDY OF THE EFFECT OF HIGH MOLECULAR WEIGHT HYALURONIC ACID IN MANAGEMENT OF TEMPOROMANDIBULAR JOINT OSTEOARTHRITIS IN RATS. test, 44(3), 89-92. doi: 10.21608/adjalexu.2019.63564
Yasmine M. Tolba; Samia S. Omar; Dina A. Nagy. "A BIOCHEMICAL STUDY OF THE EFFECT OF HIGH MOLECULAR WEIGHT HYALURONIC ACID IN MANAGEMENT OF TEMPOROMANDIBULAR JOINT OSTEOARTHRITIS IN RATS". test, 44, 3, 2019, 89-92. doi: 10.21608/adjalexu.2019.63564
Tolba, Y., Omar, S., Nagy, D. (2019). 'A BIOCHEMICAL STUDY OF THE EFFECT OF HIGH MOLECULAR WEIGHT HYALURONIC ACID IN MANAGEMENT OF TEMPOROMANDIBULAR JOINT OSTEOARTHRITIS IN RATS', test, 44(3), pp. 89-92. doi: 10.21608/adjalexu.2019.63564
Tolba, Y., Omar, S., Nagy, D. A BIOCHEMICAL STUDY OF THE EFFECT OF HIGH MOLECULAR WEIGHT HYALURONIC ACID IN MANAGEMENT OF TEMPOROMANDIBULAR JOINT OSTEOARTHRITIS IN RATS. test, 2019; 44(3): 89-92. doi: 10.21608/adjalexu.2019.63564

A BIOCHEMICAL STUDY OF THE EFFECT OF HIGH MOLECULAR WEIGHT HYALURONIC ACID IN MANAGEMENT OF TEMPOROMANDIBULAR JOINT OSTEOARTHRITIS IN RATS

Alexandria Dental Journal
Article 17, Volume 44, Issue 3, December 2019, Pages 89-92    PDF (217.74 K)
Document Type: Original Article
DOI: 10.21608/adjalexu.2019.63564
Authors
Yasmine M. Tolba* 1; Samia S. Omar2; Dina A. Nagy3
1Demonstrator of Oral Biology, Faculty of Dentistry, Alexandria University.
2Professor of Oral Biology, Faculty of Dentistry, Alexandria University.
3Lecturer of Oral Biology, Faculty of Dentistry, Alexandria University.
Abstract
INTRODUCTION: Osteoarthritis is one of the most common degenerative diseases affecting the joints. Adjuvant- induced arthritis (AIA) is an experimental model of polyarthritis which has been widely used for preclinical testing of several anti-arthritic agents and investigating the pathogenesis of arthritis. A modified model of AIA was used in this study, where the disease was induced in one joint. Hyaluronic acid deals with the degenerative effects of arthritis from 3 different aspects: mechanically by maintaining lubrication, metabolically by providing nutritional materials to the articular disc and biologically by decreasing inflammatory effects. OBJECTIVES: to study the biochemical actions of high molecular weight hyaluronic acid (HMWHA) in management of temporomandibular joint (TMJ) adjuvant induced arthritis by detecting matrix metalloproteinase-3 (MMP3) concentration in TMJ tissue extract. MATERIALS AND METHODS: 14 male albino rats were divided into 2 equal groups and in each group a split mouth design was used. •Study I: Arthritis was induced in 7 animals through a single intra-articular injection of Complete Freund's Adjuvant (CFA) in the left TMJs. (50µl) •Study II: Arthritis was induced in the other 7 animals through a single intra-articular injection of CFA and treated with an intra-articular injection of HMWHA into their left TMJs, once a week for total of three injections. 7 animals were sacrificed on day 7 of the disease while the other 7 animals were sacrificed one week after the last injection of HMWHA. Elisa test was performed to detect MMP 3 which is considered as a biomarker of the disease progression RESULTS: The concentration of MMP-3 of study I (diseased group) on day 7 was found to be significantly higher than that of study II (treated group) on day 28. CONCLUSIONS: High molecular weight hyaluronic acid is capable of management of osteoarthritic TMJs.
Keywords
TMJ; Arthritis; High molecular weight hyaluronic acid; MMP-3
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