Ex vitro Performance and Genetic Stability of Strawberry Plants Derived from Different in Vitro Propagation Methods | ||
| Hortscience Journal of Suez Canal University | ||
| Article 4, Volume 6, Issue 1, January 2017, Pages 39-50 PDF (465.05 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/hjsc.2017.6395 | ||
| Authors | ||
| Fouad Mohamed; Khalid Abd El-Hamed; Farouk Omar; Abdallah El-Shahat | ||
| Department of Horticulture, Faculty of Agriculture, Suez Canal University, Egypt 41522 | ||
| Abstract | ||
| Ex vitro experiment was conducted at the greenhouse and nursery facilities of the Plant Tissue Culture Laboratory of Department of Horticulture, Faculty of Agriculture, Suez Canal University during the period 2009-2012. Strawberry ex vitro growth performance of tissue culture-derived plants from different propagation methods (meristem, direct regeneration and callus-derived plants) were examined with standard runner-propagated mother plants based on morphological traits and genetic analysis using RAPD. Ex vitro growth performance examination showed that leaf morphology and serration patterns were almost similar among plants obtained from meristem, direct or callus regeneration. Tissue culture plants have higher number of runners while standard runner plants were higher in flower production compared with in vitro propagated plants. RAPD analysis indicated that plants of cv. 'Tudla' from the different in vitro propagation methods were genetically stable, while in cv. 'Festival', callus-derived plants deviated from normal genotype. The obtained results confirmed the usefulness of RAPD in determining genetic stability of plants clonally propagated in vitro as well as detecting the somaclonal variants, which might be used further in breeding programs. | ||
| Keywords | ||
| Fragaria x ananassa Duch; tissue culture; RAPD; genotypes; Adventitious Regeneration | ||
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