Growth Responses of Potato Plantlets Cultured In Vitro under Different Colors Light-Emitting Diodes (LEDs) | ||
| Hortscience Journal of Suez Canal University | ||
| Article 6, Volume 6, Issue 1, January 2017, Pages 65-71 PDF (223.93 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/hjsc.2017.6397 | ||
| Author | ||
| Genesia Omar | ||
| Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia - 41522, Egypt | ||
| Abstract | ||
| This exploration work target was to upgrade the micropropagation of potato cultivars through the utilization of new light sources in the development rooms. The experiment consisted of two potato cultivars (Diamant and agria), and five light sources (white, blue, green and red LEDs; and white fluorescent lights). The explants comprised of nodal segments containing one bud, segregated from plantlets developed in vitro. The experimental design was completely randomized arranged in a 2x5 factorial, with 4 replications. Each experimental unit consisted of a flask with five explants. Three 28-day successive subcultures were done in MS medium and incubated under controlled conditions (temperature = 25+2ºC; photoperiod = 16 hours; light intensity = 20 μmol m-2 s-1). Toward the finish of every subculture, plantlet length, number of nodes per plantlet, internode length and plantlet fresh weight were assessed. After the second subculture, differences were found among the two potato cultivars developed in vitro: "Agria" performed better than "Diamant" in most plantlet growth parameters. Different light sources influenced the potato plantlet development: red and green LEDs were the most and least prescribed for plantlet advancement respectively, in view of the after effects of bud number per plantlet, plantlet length. Photosynthetic pigment contents were significantly different under the different light treatments. Chlorophyll a, b and carotenoids were higher in plantlets grown under Red, and less in plantlets under green LED light. | ||
| Keywords | ||
| Solanum tuberosum L; tissue culture; microtuberization; Light | ||
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