Enany, M., Ammar, A., Maghwery, M., Gamal, N., Badr, S., Mohamed, S. (2015). Genotypic Characterization of Clostridium Perfringens and Associated Histopathological Changes in Broiler. test, 20(2), 439-447. doi: 10.21608/scvmj.2015.64650
Mohamed Enany; Ahmed Ammar; Mona Maghwery; Noha Gamal; Sohair Badr; Sohair Mohamed. "Genotypic Characterization of Clostridium Perfringens and Associated Histopathological Changes in Broiler". test, 20, 2, 2015, 439-447. doi: 10.21608/scvmj.2015.64650
Enany, M., Ammar, A., Maghwery, M., Gamal, N., Badr, S., Mohamed, S. (2015). 'Genotypic Characterization of Clostridium Perfringens and Associated Histopathological Changes in Broiler', test, 20(2), pp. 439-447. doi: 10.21608/scvmj.2015.64650
Enany, M., Ammar, A., Maghwery, M., Gamal, N., Badr, S., Mohamed, S. Genotypic Characterization of Clostridium Perfringens and Associated Histopathological Changes in Broiler. test, 2015; 20(2): 439-447. doi: 10.21608/scvmj.2015.64650

Genotypic Characterization of Clostridium Perfringens and Associated Histopathological Changes in Broiler

Suez Canal Veterinary Medical Journal. SCVMJ
Article 31, Volume 20, Issue 2, December 2015, Pages 439-447    PDF (1.38 M)
Document Type: Original Article
DOI: 10.21608/scvmj.2015.64650
Authors
Mohamed Enany1; Ahmed Ammar2; Mona Maghwery3; Noha Gamal4; Sohair Badr4; Sohair Mohamed* 5
1Microbiology Department, Fac. of Vet. Med., Suez Canal University
2Microbiology Department, Fac. of Vet. Med., Zagazig University
3Anaerobic Unit, Bacteriology Research Department, Animal Health Research Institute, Dokki, Giza
4Pathological unit, Animal Health Research Institute, Dokki, Giza
5Pathological unit, Animal Health Research Institute, Ismailia branch.
Abstract
In the present study, 6 C.perfringens strains (4 were type A and 2 were type D) were recovered from intestinal and liver samples of 6 diseased broiler. PCR  used for the detection of  alpha and epsilon toxin . Multiplex PCR was done to C.perfringens isolates which had the gene  gave a characteristic` band at 402 bp and 541 bp where all of the 6 isolateswere positive for alpha  toxin genes.The extracted toxins were purified by ammonium sulfate precipitation, then profiling of the exotoxin proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis  (SDS-PAGE).SDS-PAGE has become an important tool for protein profiling which reflect the genetic identity to C.perfringense type A and D. Our study revealed the histopathological changes of  intestinal  samples of the diseased birds with necrotic enteritis showed Intestine hyaline degeneration of the muscle, fragmentation and edema in between the muscle infiltrated with inflammatory cells. Liver showing fibrous connective tissue in the portal area particularly around intrahepatic bile duct and hyperplastic proliferation of biliary epithelium toward the lumen associated with central vein.
Keywords
genotypic; Clostridium perfringens; Histopathological Changes; broiler
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