DNA Damage in Ehrlich Carcinoma induced by Gold Nanorods Mediated Photothermal Therapy | ||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||
| Article 11, Volume 11, Issue 3, December 2019, Pages 117-126 PDF (1.36 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsc.2019.65047 | ||
| Authors | ||
| Noha Mohamed; Fatma Al-zahraa Sayed; Ahmed S. Monem; Monira M. Rageh | ||
| Biophysics Department, Faculty of Science, Cairo University, 12613 Giza, Egypt | ||
| Abstract | ||
| Gold nanorods (AuNRs) are promising nanoplatforms for cancer photothermal therapy due to their capability to absorb light in the near-infrared (NIR) window. This work was conducted to evaluate the DNA damage in Ehrlich tumor tissues associated with photothermal therapy mediated with very small sized pegylated gold nanorods (PEG-AuNRs). The accumulated gold nanorods inside tumor tissues were photo-excited with 300 mW NIR laser for 50 min. The physical properties of PEG-AuNRs were investigated using transmission electron microscopy, size distribution measurement, and UV-vis absorption spectroscopy. The efficacy of the in-vivo photothermal therapy of intravenously injected PEG-AuNRs was assessed using tumor size measurements. Also, oxidative stress and comet assay were performed to measure the percentage of DNA damage. The results showed that PEG-AuNRs were successfully prepared with a length of 8 nm and a longitudinal absorption peak at 800 nm. FTIR study confirmed the successful coating of polyethylene glycol on the surface of the gold nanorods. The in vivo experiment showed that PEG-AuNRs elicited tumoral growth arrest by producing free radicals in addition to its photo heat conversion which was sufficient to induce cellular damage by causing DNA damage | ||
| Keywords | ||
| Photothermal therapy; Cancer; DNA damage; very small Gold nanorods | ||
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