Role of cytogenetic studies, protein electrophoresis and ISSR markers in assessment of genetic diversity in Stevia rebaudiana (Bertoni). | ||
| Annals of Agricultural Science, Moshtohor | ||
| Article 39, Volume 56, 4th ICBAA - Serial Number 1, 2018, Pages 177-186 PDF (1.24 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/assjm.2018.65136 | ||
| Author | ||
| Makhlouf Bekhit* | ||
| Faculty of Agriculture at Moshtohor, Benha University | ||
| Abstract | ||
| Chromosome number was investigated in a number of strains of Stevia rebaudiana. The chromosomes were analyzed during mitosis and different stages of meiosis. All stevia strains were found to had 2n = 22. The number of bivalents at diakinesis was 11 for all the studied strains. Analysis of SDS-PAGE of soluble protein revealed complete similarity (100%) among the six strains of Stevia while two strains (broad and narrow leaves) are different in one band. Eight genotypes were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships. The level of polymorphism detected among the eight strains of stevia was 100.00% using inter simple sequence repeat (ISSR). A total of 231 clear bands were generated, out of which 34 (14.72 %) were unique bands. The total number of markers varied from 1 to 12 with a mean of 5.67 markers per primer. | ||
| Keywords | ||
| Chromosomes; Stevia; bivalents; SDS-PAG Eelectrophoresis; ISSR | ||
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