Chemical Investigation of Flavonoid, Phenolic acids Composition and Antioxidant activity of Tagetes erecta Flowers | ||
| Egyptian Journal of Chemistry | ||
| Article 16, Volume 63, Issue 7, July 2020, Pages 2605-2615 PDF (1.41 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2019.19839.2197 | ||
| Authors | ||
| Hanim A Youssef* 1; Safaa M Ali2; Mostafa I Sanad1; Dawood H Dawood3 | ||
| 1Department of agriculture chemistry, faculty of agriculture, Mansoura university | ||
| 2Department of agriculture chemistry, faculty of agriculture, Mansoura univeristy | ||
| 3Department of agriculture chemistry, faculty of agriculture,Mansoura univeristy | ||
| Abstract | ||
| This study was geared towards discovering the chemical profile and in vitro antioxidant activity of methanol (MeOH) extract and its ethyl acetate (EtOAc) fraction of Tagetes erecta orange flowers. A total of 29 known chemical compounds including phenolic acids and flavonoid compounds were identified and quantified using high performance liquid chromatography (HPLC) technique. The identification was carried out in EtOAc fraction for the first time. Pyrogallol and vanillic acid were highly abundant phenolic acids in flowers methanolic extract and its EtOAc fraction amounted 37.57 and 3.34mg/g dry extract, respectively. On the other hand, apig-6-rhamnose-8-glucose and hesperidin were found to be the major flavonoid compounds detected in MeOH extract amounted 18.34 and 0.66mg/g dry extract, respectively. Furthermore, results asserted that the tested samples were more efficient in the three applied antioxidant methods compared with standard antioxidant references (Ascorbic acid and BHT), especially EtOAc fraction which displayed the most activity against 1, 1-di phenyl-2-picrylhydrazyl (DPPH) radical with IC50 value 32.5± 0.12 µg/ml. These finding highlighted the use of Tagetes erecta flowers as a promising source of alternative bioactive compounds for both industrial and medicinal uses. | ||
| Keywords | ||
| Marigold flowers; MeOH; EtOAc; Phenolics; Flavonoids; Antioxidant activity | ||
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