Resistance Status and Associated Resistance Mechanisms to Certain Insecticides in Rice Weevil Sitophilus oryzae (Coleoptera: Curculionidae) | ||
| Alexandria Journal of Agricultural Sciences | ||
| Article 1, Volume 62, Issue 4, August 2017, Pages 331-340 PDF (731.93 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/alexja.2017.67624 | ||
| Authors | ||
| Attia Manal A.1; F. Wahba Trandil1; I. Mackled Marwa2; Shawir M. S.3 | ||
| 1Insecticide Bioassay Department, Central Agricultural Pesticides Lab. (CAPL), Agriculture Research Center (ARC), 21616, Alexandria, Egypt | ||
| 2Department of stored product Pests, Plant Protection Institute, Agriculture Research Center (ARC), Alexandria, Egypt | ||
| 33Pesticide Chemistry and technology Department, Faculty of Agriculture, Alexandria University, Elshatby, Alexandria, Egypt | ||
| Abstract | ||
| The rice weevil, Sitophilus oryzae (Coleoptera: Curculionidae) was collected from El-Behera governorate (EB). The first generation showed reduced susceptibility to deltamethrin and malathion compared with the laboratory susceptible strain (LS). Susceptibility test results (using LS colony as a reference strain) indicated that EB strain has relative toxicity ratio, RR50 and RR90 of 16.64 and 9.08 for malathion. Although, EB strain was tolerant to deltamethrin with RR50 of 5.2, it was marginally resistant to cypermethrin and entirely susceptible to permethrin. The biochemical results showed elevation in the activity of total esterase (ES), carboxylesterase (CE) and glutathione S- transferase (GST) which suggested that the metabolic resistance may has a key function in this population. Furthermore, acetylycholinesterase (AChE) activity in EB strain was 3.52 times higher compared with LS strain which pointed the insensitivity of AChE in EB strain. Moreover, esterase analysis indicated genetic polymorphism between EB and LS populations which might be attributed to selection pressure due to continuous exposure to insecticides. This study suggested the existence of malathion and deltamethrin resistance in EB strain which might be a consequence of biochemical alterations. The results of current study indicate the importance of continuous monitoring of resistance of stored product insect pests to plan successful management strategies. | ||
| Keywords | ||
| insecticide resistance; metabolic resistance; esterase; Sitophilus oryzae; malathion | ||
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