Mikawye, E., Tolba, S., Ezat, A., Unis, A., Ibrahim, M. (2017). Antimicrobial activity of methanolic Nigella sativa seeds and Saussurea lappa roots extracts on multidrug resistant Gram negative bacteria. test, 13(1), 155-163. doi: 10.21608/ajbs.2017.68644
Eslam Mikawye; Sahar Tolba; Amgad Ezat; Amina Unis; Mohamed Ibrahim. "Antimicrobial activity of methanolic Nigella sativa seeds and Saussurea lappa roots extracts on multidrug resistant Gram negative bacteria". test, 13, 1, 2017, 155-163. doi: 10.21608/ajbs.2017.68644
Mikawye, E., Tolba, S., Ezat, A., Unis, A., Ibrahim, M. (2017). 'Antimicrobial activity of methanolic Nigella sativa seeds and Saussurea lappa roots extracts on multidrug resistant Gram negative bacteria', test, 13(1), pp. 155-163. doi: 10.21608/ajbs.2017.68644
Mikawye, E., Tolba, S., Ezat, A., Unis, A., Ibrahim, M. Antimicrobial activity of methanolic Nigella sativa seeds and Saussurea lappa roots extracts on multidrug resistant Gram negative bacteria. test, 2017; 13(1): 155-163. doi: 10.21608/ajbs.2017.68644

Antimicrobial activity of methanolic Nigella sativa seeds and Saussurea lappa roots extracts on multidrug resistant Gram negative bacteria

African Journal of Biological Sciences
Article 14, Volume 13, Issue 1, 2017, Pages 155-163    PDF (551.81 K)
Document Type: Original Article
DOI: 10.21608/ajbs.2017.68644
Authors
Eslam Mikawye* 1; Sahar Tolba2; Amgad Ezat3; Amina Unis4; Mohamed Ibrahim2
11- Research, Studies and Training Center on vectors of diseases, Faculty of Science, Ain Shams Univ
2Department of Microbiology, Faculty of Science, Ain Shams University.
3Department of Microbiology and Immunology, Faculty of Medicine, Al-Azhar Univ., Assuit, Egypt
4Department of pharmacology, Faculty of Medicine, Alexandria. University, Egypt
Abstract
Different solvent extracts of Nigella sativa seeds and Saussurea lappa roots were investigated for their antimicrobial activity against some Gram negative bacteria isolated from clinical samples from neonatal intensive care unit (NICU) from King Khalid Hospital, Tabuk, Kingdom of Saudi Arabia.Ten clinical isolates, Pseudomonas aeruginosa (n=5), Serratia marcescens (n=2), Proteus mirabilis (n=2) and Sphingomonas paucimobilis (n=1). These isolates were multidrug resistant. The methanolic extract of S. lappa showed high antimicrobial activity rather than that of Nigella sativa seeds especially against Sphingomonas sp. and Serratia sp.. Both methanolic extracts of  S. lappa roots and Nigella sativa seeds showed antibacterial activity against Pseudomonas sp. and Proteus sp with varying extents. The chemical composition of the investigated two plant extracts was examined. It contained phenols, tannins and flavenoids. The emergence of antibiotic resistance and the high risk of the abuse of antibiotics, highlight the need of using alternative agents that have low risk and costless.
Keywords
Antibacterial activity; Nigella Sativa; Saussurea lappa; methanolic extract; Grame negative bacteria
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