IN VITRO AND IN VIVO OVULATORY RESPONSE OF RABBIT DOES TREATED WITH GnRH OR PMSG. | ||
| Journal of Animal and Poultry Production | ||
| Article 4, Volume 5, Issue 5, May 2014, Pages 279-291 PDF (966.51 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jappmu.2014.69557 | ||
| Authors | ||
| A. Z. Mehrez,1; M. A. Moustafa1; Sh. M. Shamiah2; Noha F. Abd El Raouf1 | ||
| 1Anim. Prod. Dept., Fac. Agric., Mansoura University. | ||
| 2Anim. Prod. Res. Inst., Ministry of Agric. | ||
| Abstract | ||
| Twenty four New Zealand White (NZW) rabbit does (5-7 mo of age, 2.5-4.6 kg LBW and 1-2 parities) were used to study the effect of induced ovulation by GnRH or PMSG on ovarian characteristics, recovery and measurements of embryos at different stages. Also, 3 fertile NZW bucks were used for natural mating. Rabbit does were divided into 3 groups, (8 does in each). Does in the first group (G1) were injected (i.m.) with 0.2 ml saline solution/doe immediately before mating (control). Does in G2 and G3 were i.m. injected with 35 IU PMSG/doe 48 h before mating and 0.2 ml GnRH/doe at mating, respectively. Ovarian and genital characteristics including weight, length and width of the ovaries, oviduct and uterine horn were determined on the right and left side. Embryos were recovered by flushing from each treated doe slaughtered after 72 h of mating for collection of embryos.Embryos were recovered from each uterine horn and oviduct per doe. Results showed that all ovarian characteristics were not affected by treatment, ovarian side and their interaction.Ovulatory response in terms of average number of bleeding and total follicles, corpora lutea (CLs) not significantly affected by treatment. Rabbit does in G3 significantly (P<0.05) produced the greatest number of embryos/doe and the highest blastocyst rate in vitro, and the highest kindling rate, litter size and viability rate of bunnies. In conclusion, treatment of NZW doe rabbits with 0.2 ml GnRH/doe (Receptal) at mating may be efficient in in vivo embryo production for embryo transfer and also needed for increasing in vivo fertility of naturally mated rabbit does. | ||
| Keywords | ||
| Rabbit; PMSG; GnRH; ovarian characteristics; litter size; in vivo fertility | ||
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