Aly, S., Eisa, A., ElBanna, N. (2019). Characterization of Vibrio Alginolyticus Infection in Gilthead Seabream (Sparus Auratus, L) Cultured in Egypt. test, 24(2), 287-301. doi: 10.21608/scvmj.2019.69851
Salah Aly; Alaa Eisa; Noha ElBanna. "Characterization of Vibrio Alginolyticus Infection in Gilthead Seabream (Sparus Auratus, L) Cultured in Egypt". test, 24, 2, 2019, 287-301. doi: 10.21608/scvmj.2019.69851
Aly, S., Eisa, A., ElBanna, N. (2019). 'Characterization of Vibrio Alginolyticus Infection in Gilthead Seabream (Sparus Auratus, L) Cultured in Egypt', test, 24(2), pp. 287-301. doi: 10.21608/scvmj.2019.69851
Aly, S., Eisa, A., ElBanna, N. Characterization of Vibrio Alginolyticus Infection in Gilthead Seabream (Sparus Auratus, L) Cultured in Egypt. test, 2019; 24(2): 287-301. doi: 10.21608/scvmj.2019.69851

Characterization of Vibrio Alginolyticus Infection in Gilthead Seabream (Sparus Auratus, L) Cultured in Egypt

Suez Canal Veterinary Medical Journal. SCVMJ
Article 13, Volume 24, Issue 2, December 2019, Pages 287-301    PDF (689.69 K)
Document Type: Original Article
DOI: 10.21608/scvmj.2019.69851
Authors
Salah Aly1; Alaa Eisa2; Noha ElBanna* 3
1Department of Pathology-Faculty of Veterinary Medicine-Suez Canal Univ
2Department of Aquaculture Animal Medicine & Management-Faculty of Veterinary Medicine-Cairo Univ.
3Department of Aquaculture Diseases Control-Fish Farming & Technology Institute-SCU
Abstract
The present study investigated Vibriosis in cultured Gilthead Seabream at the Suez Canal area, Egypt. Two hundred moribund fish samples subjected to clinical, microbiological and histopathological examinations. Retrieved isolates were biochemically identified using API 20 E system then confirmed by conventional PCR. Antibiogram profiling of the retrieved isolates was also done.Naturally infected fish showed severe ascites, hemorrhages and erosions in the skin and fins.A sum of 29 V. alginolyticus isolates were retrieved from the examined samples. Targeting 16SrRNA and groEl genes produce an expected product with a length of 336, 301bp size bands; respectively, were found from all tested strains of V. alginolyticus. The antibiogram profiling of V. alginolyticus isolates showed resistance to Ampicillin, Gentamycin followed by Ciprofloxacin with high sensitivity to Sulphamethoxazole/Trimethoprim and oxytetracycline. Histopathological alterations in the affected tissues showed, inflammatory reactions together with degenerative and /or necrotic changes in branchial, liver and kidney.
Ultimately, the current study emphasizes the critical fish health threats posed by V. alginolyticus with subsequent influences on human. Thus, competent biosecurity strategies should be adopted to control Vibrio infection in marine fishes and to minimize the antimicrobial resistance hazards in mariculture.
Keywords
Vibrio alginolyticus; Gilthead seabream; 16s rRNA gene; groEl gene; antibiogram and histopathology
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