Abdelkader, M., Shawky, N., Khalaf AKL, H., Bahgat, M. (2022). Helicobacter pylori BabA2 ,CagA and VacA genes ; A new paradigm for gastric lesion and bacterial carcinogenesis. test, 28(5), 930-939. doi: 10.21608/zumj.2020.16066.1562
Mirham Abdelkader; Nagwa Shawky; Hosnia Khalaf AKL; Maged Bahgat. "Helicobacter pylori BabA2 ,CagA and VacA genes ; A new paradigm for gastric lesion and bacterial carcinogenesis". test, 28, 5, 2022, 930-939. doi: 10.21608/zumj.2020.16066.1562
Abdelkader, M., Shawky, N., Khalaf AKL, H., Bahgat, M. (2022). 'Helicobacter pylori BabA2 ,CagA and VacA genes ; A new paradigm for gastric lesion and bacterial carcinogenesis', test, 28(5), pp. 930-939. doi: 10.21608/zumj.2020.16066.1562
Abdelkader, M., Shawky, N., Khalaf AKL, H., Bahgat, M. Helicobacter pylori BabA2 ,CagA and VacA genes ; A new paradigm for gastric lesion and bacterial carcinogenesis. test, 2022; 28(5): 930-939. doi: 10.21608/zumj.2020.16066.1562

Helicobacter pylori BabA2 ,CagA and VacA genes ; A new paradigm for gastric lesion and bacterial carcinogenesis

Zagazig University Medical Journal
Article 4, Volume 28, Issue 5, September 2022, Pages 930-939    PDF (380.6 K)
Document Type: Original Article
DOI: 10.21608/zumj.2020.16066.1562
Authors
Mirham Abdelkader* 1; Nagwa Shawky2; Hosnia Khalaf AKL3; Maged Bahgat4
1department of cliniical pathology , zagazig university hospitals,egypt
2clinical pathology department , zagazig university hospitals,Egypt
3clinical pathology department, faculty of medicine, zagazig university
4tropical medicine , faculty of medicine, zagazig university
Abstract
Background: Helicobacter pylori (H pylori) is a major etiological agent in several gastroduodenal diseases. The most common virulence markers of H pylori are vacuolating cytotoxin (VacA), cytotoxin associated gene A (CagA), and blood adhesion binding antigen (BabA).
Objective: The study aimed to investigate the relation of VacA, CagA and BabA2 genotypes of H pylori strain to the endoscopic findings particularly (peptic ulcer or gastric carcinoma) in infected patients.
Patients, Materials and Methods: A cross sectional study was carried out in the clinical pathology department and Tropical Endoscopy unit at Faculty of medicine, Zagazig university..The study included 100 patients who attended the Endoscopy unit for Upper gastrointestinal endoscopy due to different complains (epigastric pain, heart burn, regurgitation, dyspepsia and vomiting. Two gastric biopsy samples were taken for Rapid Urease Test (RUT) and PCR.

Results: The study showed 62 out of hundred patients (62%) were H.pylori positive and 50 out of 62 H pylori positive cases (81%) revealed one or more virulent factor. The positive rate of VacA, CagA and BabA2 among H.pylori positive patients were 73%.23% and 26% respectively. The allelic variant Vac s2m2 was more prevalent 42% than other alleles. The most predominant genotype combination VacA/CagA 7/50 (14%) . VacA was found among 49% in cases of peptic ulcer. A statistically significant different between VacA, triple gene combination and the clinical outcome. P
Keywords
Helicobacter pylori; CagA; BabA2; VacA; gastric lesions
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