OPTIMIZATION OF AGITATION AND INCUBATION PERIOD ON PRODUCTION OF MANNANASE BY BACILLUS VELEZENSIS NRC-1 USING BENCH-SCALE BIOREACTOR | ||
| Al-Azhar Journal of Pharmaceutical Sciences | ||
| Article 18, Volume 45, Issue 1 - Serial Number 45, March 2012, Pages 265-273 PDF (517.93 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajps.2012.7243 | ||
| Author | ||
| Tarek Mazeed | ||
| Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza 12622, Egypt. | ||
| Abstract | ||
| The effect of agitation speed and incubation period on the production of mannanase enzyme by Bacillus velezensis NRC-1 using bench-scale bioreactor at 45ºC was investigated. Results revealed that the increase in agitation speed from 200 to 800 rpm resulted in a significant increase in mannanase production to 21.04 U/mL. The increase in agitation speed affected dissolved oxygen (DO) concentration which in turn affected cell growth and mannanase production. The maximum mannanase production in the bioreactor was attained after 72 h of incubation at 45 ºC. Mannanase activity in the bioreactor (21.04 U/mL) while much higher than that obtained from the shake flask fermentation (15.6 U/mL) also the incubation period decreased from 7 days in shake flask fermentation to 3 days in bioreactor. | ||
| Keywords | ||
| Bacillus velezensis; Mannanase; shaking; Bioreactor | ||
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