DNA Fingerprinting and Characterization of some Egyptian Date Palm Cultivars Using Simple Sequence Repeats (SSRs) | ||
| Journal of Plant Production Sciences | ||
| Article 4, Volume 3, Issue 1, June 2015, Pages 31-41 PDF (1.47 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpps.2015.7402 | ||
| Authors | ||
| Mohammed Hussein; Samah Sabry; Manal Eid* | ||
| Botany Department, Faculty of Agriculture, Suez Canal University, 41522. Ismailia. Egypt | ||
| Abstract | ||
| The Date Palm (Phoenix dactylifera L), germplasm commonly cultivated in Egypt, shows a wide range of repining periods and fruit quality and is an unexploited resource for breeding programs. The main purpose of this study was to fingerprint 45 date palm genotypes and accessions and to construct a molecular database including the cultivars commonly grown in Egypt. An analysis of thirty three microsatellite simple sequence repeat (SSR) loci out of thirty five markers was performed to define distinct specific alleles across all loci, genetic similarity within cultivars in each region of five different locations and possibility of linking morphological traits with molecular data. In general, the results indicated the possible use of SSR analysis to detect cultivar-specific markers for forty five Date palm cultivars and accessions under investigation that can be used to discriminate among the cultivars and genotypes. The genetic distance among populations compared to each other (Hayani, Samany and Zagholol) which presented at different locations in this study, may or may not correlate with the geographical distance between them in some species, depending on natural and artificial factors involved in shaping the population genetic structure of the species. Based on our findings, it is clear that there is some discordance between the data corresponding to molecular variability and those related to the variability available for breeding purposes (phenotypic variability). However, the use of 33 polymorphic microsatellite markers to study Egyptian date palm germplasm suggested that this is reliable, efficient and effective marker system that can be used for diversity analysis, and subsequently in crop improvement programs. | ||
| Keywords | ||
| Microsatellite loci; Specific alleles; Genetic Distance; Morphological traits | ||
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