Effects of Erythropoietin on The Healing of Calvarial Bone Defect | ||
| Egyptian Dental Journal | ||
| Article 2, Volume 65, Issue 4 - October (Oral Surgery), October 2019, Pages 3283-3294 PDF (3.46 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/edj.2019.74758 | ||
| Authors | ||
| Alshaimaa Ahmed1; Inass Abou Elmagd2; Maggie Ahmed Khairy3; Ahmed Nabil Fahmi4 | ||
| 1Associate Professor Oral & Maxillofacial Surgery, Faculty of Dentistry, Fayoum University, Egypt | ||
| 2Associate Professor Radiology, Faculty of Dentistry, Fayoum University, Egypt | ||
| 3Associate Professor Oral & Maxillofacial Surgery, Faculty of Dentistry, 6th October University, Egypt | ||
| 4Associate Professor Oral Pathology, Faculty of Dentistry, Bani Suif University, Egypt | ||
| Abstract | ||
| Objectives To evaluate the effect of a single-dose local administration versus the systemic administration of Erythropoietin on bone healing in calvarial bone defects Material & Methods Critical-size cranial osteotomy defects were created in 30 rabbits. The animals were randomly divided into 3 groups. The animals were randomly divided into 3 groups (n= 10 animals in each group). In the Group I, the bone defect was only filled with a collagen sponge soaked with erythropoietin. In the Group II, it was filled with a collagen sponge and erythropoietin injected systemically. While in the Group III, the defect was filled with a collagen sponge. The groups were further split in two for euthanasia 10- and 21-days post-surgery. New bone formation and neovascularization were evaluated by hematoxylin-eosin. For the 10-days samples, all the groups analyzed for area percent of blood vessels while on the 21- day samples, the area of new bone formation was calculated. Differences between groups were analyzed by one-way ANOVA. Results At 10 days post-surgery, the histological analysis showed that the erythropoietin Groups exhibited a significantly higher percentage of bone formation compared with the other Group. At 21 days post-surgery, a higher percentage of new bone was observed in the erythropoietin group. Conclusions The results suggest that both local and systemic administration of erythropoietin hormone encouraged the bone healing in critical-size calvarial defects in Rabbits. | ||
| Keywords | ||
| erythropoietin; calvarial bone defect; Rabbits; topical dose | ||
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