Atwa, D., Hussein, M., El-Khawas, M., Sarhan, A., Mandour, N., Mahmoud, M. (2017). The Effect of some Insecticides on Two Entomopathogenic Nematodes and Evaluation of Its Virulence on the Greater Wax Moth Galleria mellonella. test, 6(1), 37-45. doi: 10.21608/japp.2017.7578
Dina Atwa; Mona Hussein; Mostafa El-Khawas; Awad Sarhan; Nasser Mandour; Mahmoud Mahmoud. "The Effect of some Insecticides on Two Entomopathogenic Nematodes and Evaluation of Its Virulence on the Greater Wax Moth Galleria mellonella". test, 6, 1, 2017, 37-45. doi: 10.21608/japp.2017.7578
Atwa, D., Hussein, M., El-Khawas, M., Sarhan, A., Mandour, N., Mahmoud, M. (2017). 'The Effect of some Insecticides on Two Entomopathogenic Nematodes and Evaluation of Its Virulence on the Greater Wax Moth Galleria mellonella', test, 6(1), pp. 37-45. doi: 10.21608/japp.2017.7578
Atwa, D., Hussein, M., El-Khawas, M., Sarhan, A., Mandour, N., Mahmoud, M. The Effect of some Insecticides on Two Entomopathogenic Nematodes and Evaluation of Its Virulence on the Greater Wax Moth Galleria mellonella. test, 2017; 6(1): 37-45. doi: 10.21608/japp.2017.7578

The Effect of some Insecticides on Two Entomopathogenic Nematodes and Evaluation of Its Virulence on the Greater Wax Moth Galleria mellonella

Journal of Applied Plant Protection
Article 6, Volume 6, Issue 1, December 2017, Pages 37-45    PDF (374.71 K)
Document Type: Original Article
DOI: 10.21608/japp.2017.7578
Authors
Dina Atwa1; Mona Hussein2; Mostafa El-Khawas1; Awad Sarhan3; Nasser Mandour3; Mahmoud Mahmoud3
1Biological Control Department, Plant Protection Institute, Agricultural Research Center, Giza, Egypt.
2Department of Pests and Plant Protection, Agricultural Division, National Research Center, Dokki, Giza, Egypt.
3Plant Protection Department, Faculty of Agriculture, Ismailia, 41522, Suez Canal University, Egypt.
Abstract
The toxicity effect of five insecticides (Dipel 2x, Radiant, Proclaim, Aphox and Coragen) on two species of
entomopathogenic nematodes (EPNs) (Heterorhabditis bacteriophora BA1 and Steinernema carpocapsae BA2), were
evaluated under laboratory conditions at different concentrations 100%, 50% and 25% of field rates (FR). Moreover, the
virulence of insecticide-treated EPNs against the lst nymphal instar of the greater wax moth Galleria mellonella L.
(Lepidoptera: Pyralidae) was also evaluated. Data indicated that all insecticides showed negative effect on the tested
infective juveniles (IJs) of EPNs and the percentages of mortality were in order of H. bacteriophora BA1>S.
carpocapsae BA2 and were concentration-dependent; significant differences existed between untreated (control) and
insecticide treatments EPNs. Proclaim insecticide recorded the highest mortality rate in EPNs at all tested
concentrations 24, 48 and 72 hours post treatment. Regarding the effect of tested insecticides on the virulence and the
reproductive capacity of treated EPNs, data indicated that the ability of insecticide-treated EPNs to locate and infect
larvae of G. mellonella decreased after exposure to insecticide and consequently reproductive capacity of EPNs was
significantly reduced.
Keywords
Insecticides; Steinernema carpocapsae; Heterorhabditis bacteriophora; toxicity; Galleria mellonella; Virulence
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