Khaphagy, A., Salama, S., Ibrahim, A., Hamouda, R. (2014). Molecular characterization of Listeria monocytogenes isolated from milk. test, 19(1), 17-24. doi: 10.21608/scvmj.2014.76090
Ahmed Khaphagy; S. Salama; A. Ibrahim; R. Hamouda. "Molecular characterization of Listeria monocytogenes isolated from milk". test, 19, 1, 2014, 17-24. doi: 10.21608/scvmj.2014.76090
Khaphagy, A., Salama, S., Ibrahim, A., Hamouda, R. (2014). 'Molecular characterization of Listeria monocytogenes isolated from milk', test, 19(1), pp. 17-24. doi: 10.21608/scvmj.2014.76090
Khaphagy, A., Salama, S., Ibrahim, A., Hamouda, R. Molecular characterization of Listeria monocytogenes isolated from milk. test, 2014; 19(1): 17-24. doi: 10.21608/scvmj.2014.76090

Molecular characterization of Listeria monocytogenes isolated from milk

Suez Canal Veterinary Medical Journal. SCVMJ
Article 3, Volume 19, Issue 1, June 2014, Pages 17-24    PDF (394.8 K)
Document Type: Original Article
DOI: 10.21608/scvmj.2014.76090
Authors
Ahmed Khaphagy1; S. Salama2; A. Ibrahim1; R. Hamouda* 3
1Dept. of Bacteriology, Immunology and Mycology, Faculty of Vet. Med. Suez Canal University.
2Central Laboratory for Evalution of Veterinary biologics, Abbasia, Cairo
3Animal Reproductive Research Institute in Giza
Abstract
A total of 560 various raw milk samples were collected from different dairy farms to examine the presence of L.monocytogenes. These isolates were further characterized by biochemical tests and Polymerase Chain Reaction (PCR). L.monocytogenes was isolated in 72 of the 560 samples (12.9%). Based on PCR, All the examined L.monocytogenes were positive for Listeriolysin O gene (hly A) and P60 (iap).The findings of this study show that consumption of raw milk could be a potential risk of human listeriosis. 
Keywords
Molecular characterization; Listeria monocytogenes; Milk
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