Molecular characterization of Staphylococcus aureus isolated from meat, milk and their products | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Article 13, Volume 19, Issue 1, June 2014, Pages 137-152 PDF (739.67 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2014.76373 | ||
| Authors | ||
| Mahmoud Ezzat1; Iman Shabana1; Emad Riad2; Sarah Abbas* 3 | ||
| 1Department of Bacteriology, Immunology, Mycology - Faculty of Veterinary Medicine, Suez Canal University | ||
| 2Animal Health Research Institute, El-Dokki | ||
| 3Department of Bacteriology, Animal Health Research Institute, PortSaid branch | ||
| Abstract | ||
| This study was planned to throw the light on the incidence of S. aureus in 485 random samples of meat, milk and their products, The bacteriological examination of 200 meat and meat products revealed that the prevalence of S. aureus was 19 isolates (9.5%), The bacteriological examination of 285 milk and milk product revealed that the prevalence of S. aureus was16 isolates (18.8%). The prevalence of S. aureus in examined milk collected from (farms, dairy shops and street vendors) were (16%, 16% and 22.8%) respectively, While milk products showed that, the prevalence of S. aureus was 8 isolates (4%). All isolated strains were identified as S. aureus by using specific culture media and biochemical tests. Detection of enterotoxin in S. aureus isolates proved that, 27out of 43 of them were entrotoxignic , 15 isolates out of 27 were enterotoxin A producer,7 isolates were enterotoxin C producer and 5 isolates were harbored the type (A and C) . PCR used for confirmation of S. aureus isolates, revealed that S. aureus had four DNA fragments patterns of 410, 740, 910 and 970 b.p. with primer (1) meanwhile with primer (2), the pattern of S. aureus had two bands of 562 and 928 b.p. Studying the antibiotic sensitivity test of S. aureus for 15 different types of antibiotics in lab, the result revealed that most strains which isolated from meat, milk and their products were show highly degree of resistant to Methicillin followed by Oxacillin, Chloramphenicol, Amoxiciilin, Cephranin, Cephalothin, Amikacin, Gentamycin, Ciprofloxacin, doxycycline while they were sensitive to Cefaclor, Streptomycin, Norfloxacin, Erythromycin and trimethoprim- sulphamethoxazole. PCR also, confirmed the presence of mec A gene in 4 isolates (methicillin resistant S. aureus). | ||
| Keywords | ||
| Molecular characterization; Staphylococcus aureus; meat; Milk; products | ||
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