Studying the effect of gamma irradiation on bovine offal's infected with Mycobacterium tubercullosis bovine type | ||
| Veterinary Medical Journal (Giza) | ||
| Article 3, Volume 63, Issue 4, October 2017, Pages 17-24 PDF (834.03 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2017.7645 | ||
| Authors | ||
| Shaltout A1; Riad M.2; T. Ahmed3; Asmaa A.4 | ||
| 1Faculty of Veterinary Medicine, Food Control Dept. Benha Univ | ||
| 2Animal Health Research Institute, | ||
| 3Radioisotope Dept. Nuclear. Research Center. Atomic Energy Authority | ||
| 4General Organization for Veterinary services | ||
| Abstract | ||
| The presence of microbial pathogens on human foods is a serious global Problem even in highly industrialized and developed countries. The awareness of foodborne diseases by consumers will increase, and therefore, there is a pressure to improve the safety of the food supply. Gamma ray is highly effective in inactivating microorganisms in various foods and offers a safe alternative method of food decontamination. In the present study, a total of 35 samples from T.B. infected carcasses (15 samples of offal's ((7) liver & (8) Kidney) and 15 samples from different lymph nodes((10) Hepatic & (5) Renal)) were collected from some governmental Egyptian abattoirs confirmed to be infected with Mycobacterium tubercullosis bovine type by Real Time PCR were experimental treated with 0.0, 2.5, 5 and 7.5 KGy of gamma rays then, reexamined using RT-PCR for Mycobacterium tubercullosis bovine type infection. The results indicated that the reduction rate is decreased by increase the dose level of Gamma rays. At 0.0 kGy all samples still 100 % infected & 46.6% still infected at 2.5 kGy & 20 % still infected at 5 kGy and, At 7.5 % all examined samples are failed to be detected of T.B. Infected offal's. More ovever, the examined samples of T.B. Infected lymph nodes showed that at 0 kGy all samples still 100 % infected & 53.3 % still infected at 2.5 kGy & 13.3 % still infected at 5 kGy and, at 7.5 % all examined samples are free from mycobacterium infection. The effect on (Color & Odor & Texture) parameter after exposure to Gamma rays on T.B. infected samples proved that most of tested samples have slight changes in color (pale color), odor (characteristic odor of irradiation) and texture (friable) in the first 24 hours and all tested samples have been returned back into the normal parameter after 1 week. The results of the present study showed that it's advisable to use the Gamma irradiation for saving a huge amount of condemned meat due to T.B infected cattle carcasses and using it as low grade meat. | ||
| Keywords | ||
| mycobacterium bovis; bovine offal's; Gamma Irradiation | ||
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