Ashour, W., Mohammed, R., Abou Zeid, A., Sabry, O., El Hawary, S. (2020). Saraca indica L. leaves, Phytochemical, invitro Antioxidant and Cytotoxic Activities. test, 63(10), 3779-3790. doi: 10.21608/ejchem.2020.23139.2371
wedian Ashour; Reda Mohammed; Aisha H. Abou Zeid; Omar Sabry; Seham Salah Eldin El Hawary. "Saraca indica L. leaves, Phytochemical, invitro Antioxidant and Cytotoxic Activities". test, 63, 10, 2020, 3779-3790. doi: 10.21608/ejchem.2020.23139.2371
Ashour, W., Mohammed, R., Abou Zeid, A., Sabry, O., El Hawary, S. (2020). 'Saraca indica L. leaves, Phytochemical, invitro Antioxidant and Cytotoxic Activities', test, 63(10), pp. 3779-3790. doi: 10.21608/ejchem.2020.23139.2371
Ashour, W., Mohammed, R., Abou Zeid, A., Sabry, O., El Hawary, S. Saraca indica L. leaves, Phytochemical, invitro Antioxidant and Cytotoxic Activities. test, 2020; 63(10): 3779-3790. doi: 10.21608/ejchem.2020.23139.2371

Saraca indica L. leaves, Phytochemical, invitro Antioxidant and Cytotoxic Activities

Egyptian Journal of Chemistry
Article 17, Volume 63, Issue 10, October 2020, Pages 3779-3790    PDF (824.65 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2020.23139.2371
Authors
wedian Ashour1; Reda Mohammed* 2; Aisha H. Abou Zeid3; Omar Sabry4; Seham Salah Eldin El Hawary5
1Department of Pharmacognosy, National Research Centre
2Pharmacognosy Dept. , National Research Centre; 33-Elbohouth St, (Former El-Tahrir St.), Dokki – Giza-Egypt. P.O.12622, ID: 60014618
3Pharmacognosy Department, Pharmaceutical and Drug Industry, National Research Center
4pharmacognosy Dept. Faculty of pharmacy Cairo university
5Pharmacognosy Department, Faculty of Pharmacy, Cairo University
Abstract
Saraca indica L. (Saraca asoca) is reported for its important biological benefits in Indian folk medicine. The powdered leaves were extracted with 70% ethanol to obtain total ethanol extract(TEE) that was successively fractionated to get petroleum ether(pet. ether),chloroform(CHCl3), ethyl acetate (EtOAc) and methanol extracts.The antioxidant activityof TEE by three methods of assay, as well as,the antioxidant activity of TEE and successive extracts by DPPHmethod at two dose were done.Total phenolics and total flavonoids content of the plant were determined. The cytotoxic evaluation revealed that EtOAc extract exhibited activity against HCT-116 andMCF-7, Hela& HEPG-2 . GC/MS analysis of the unasponifiable(USF) and saponifiable (SF)fractions of the non-polar extractwas carried out. 34 Compounds with phytol, dodecane, BHT and α -amyrin as the major compoundsin the USFand 25 compounds with methyl palmitate&Methyl-9, 12,15- octadecatrienoate as major compounds in the SF were identified. Fifteen phenolic compounds were detected in HPLC analysis of EtOAcextract as well as six phenolic compounds were isolated and identified for the first time from the same extract they were; gallic acid, methylgallate, rutin, quercetin -3-O-rhamnoside, quercetin and kaempeferol
Keywords
Saraca indica L; antioxidant; Cytotoxic activities; Total phenolic; Total flavonoids; Unsaponifiable; Saponifiable fractions
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