Phenotypic and Genotypic Identification of Vancomycin Resistant Enterococci from Different Sources | ||
| Zagazig Veterinary Journal | ||
| Article 8, Volume 45, Issue 1, March 2017, Pages 64-73 PDF (302.71 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zvjz.2017.7688 | ||
| Authors | ||
| Adel M. Attia1; Ahlam A. Gharib1; Ibrahim I. Mohamed2; Omnia E. Ahmed* 3 | ||
| 1Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt | ||
| 2Food Hygiene Department, Animal Health Research Institute (AHRI ARC 60019332), Zagazig Provincial Lab | ||
| 3Microbiology Department, Animal Health Research Institute (AHRI ARC 60019332), Zagazig Provincial Lab | ||
| Abstract | ||
| Enterococci are reservoirs for transmission of the most clinically important antimicrobial resistances such as vancomycinresistance. Therefore, this work aimed to determine the occurrence of enterococci and their respective vancomycine resistance genes (vanA and vanB) from different sources. Two hundred and twenty-four samples from chickens, turkey, fish and human urine, as well as, two types of human food including milk (raw and milk from mastitic animals) and sausage were tested for isolation of Enterococcus species. The isolates wereidentified morphologically and biochemically usingcatalase test, sodium chloride tolerance and growth at pH 9.6and 10- 45˚C. The vancomycin resistance profile of the isolates was verified by both disc diffusion and agar dilution methods. The genotypic enterococcal identification at both genus and species levels and their vancomycine resistance genes were also ascertained using PCR amplification of the respective genes for 28 isolates. Enterococci isolation rate was 70% of the examined samples with a higher percentage of vancomycine resistance (53.5%) and the minimum inhibitory concentrations (MICs) ranged from 16 to 512 µg/mL. Molecular identification of 28 enterococcal isolates revealed the dominance of E. faecalis (42.8%) and clarified a higher proportion of vanA (78.5%) and vanB (67.8%) genes. In conclusion, administration of the antimicrobials mainly vancomycin may be considered as a pronounced stress factor in the veterinary and human practices. In addition, VRE can act as a reservoir for vancomycin resistance. | ||
| Keywords | ||
| Antimicrobial susceptibility; Vancomycin resistance; enterococci | ||
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