FARAG, S., ADEL HUSSEIN, M., HAFEZ, S., KHALED, A., KAMEL, O., ZYAAN, O. (2018). ULTRA-STRUCTURAL STUDIES ON THE MIDGUT OF CULEX PIPIENS LARVAE TREATED WITH POMEGRANATE PEEL EXTRACT, PUNICA GRANATUM. test, 48(1), 77-84. doi: 10.21608/jesp.2018.77027
SHAIMAA MAHMOUD FARAG; MOHAMED ADEL HUSSEIN; SORIYA ELTANTAWY HAFEZ; AMANY SOLIMAN KHALED; OMNIA MOHAMED HELMY MOHAMED KAMEL; OLA HELMY ZYAAN. "ULTRA-STRUCTURAL STUDIES ON THE MIDGUT OF CULEX PIPIENS LARVAE TREATED WITH POMEGRANATE PEEL EXTRACT, PUNICA GRANATUM". test, 48, 1, 2018, 77-84. doi: 10.21608/jesp.2018.77027
FARAG, S., ADEL HUSSEIN, M., HAFEZ, S., KHALED, A., KAMEL, O., ZYAAN, O. (2018). 'ULTRA-STRUCTURAL STUDIES ON THE MIDGUT OF CULEX PIPIENS LARVAE TREATED WITH POMEGRANATE PEEL EXTRACT, PUNICA GRANATUM', test, 48(1), pp. 77-84. doi: 10.21608/jesp.2018.77027
FARAG, S., ADEL HUSSEIN, M., HAFEZ, S., KHALED, A., KAMEL, O., ZYAAN, O. ULTRA-STRUCTURAL STUDIES ON THE MIDGUT OF CULEX PIPIENS LARVAE TREATED WITH POMEGRANATE PEEL EXTRACT, PUNICA GRANATUM. test, 2018; 48(1): 77-84. doi: 10.21608/jesp.2018.77027

ULTRA-STRUCTURAL STUDIES ON THE MIDGUT OF CULEX PIPIENS LARVAE TREATED WITH POMEGRANATE PEEL EXTRACT, PUNICA GRANATUM

Journal of the Egyptian Society of Parasitology
Article 9, Volume 48, Issue 1, April 2018, Pages 77-84    PDF (1.13 M)
Document Type: Original Article
DOI: 10.21608/jesp.2018.77027
Authors
SHAIMAA MAHMOUD FARAG1; MOHAMED ADEL HUSSEIN1; SORIYA ELTANTAWY HAFEZ1; AMANY SOLIMAN KHALED1; OMNIA MOHAMED HELMY MOHAMED KAMEL2; OLA HELMY ZYAAN1
1Departments of Entomology, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.
2Applied Chemistry, National Research Center, Giza, Egypt.
Abstract
The peel powder of Punica granatum, extracted with petroleum ether, was proved to have potential toxicological effects against third instar larvae of Culex pipiens. The median lethal dose (LC50) value was found to be 95.6632 ppm. Qualitative phytochemical screening of pomegranate peel extract was assessed by standard methods. The phytochemical constituents present in petroleum ether extract of Punica granatum peel were phenols and Saponins. The mid gut of the treated larvae was investigated histopathologically and ultra-structurally. The tested extract induced a severe damage to the larval midgut epithelial cells; showing swollen, lysed and displayed clear destruction in the peritrophic membrane and brush border. Also, several changes in cell organelles including destruction of the cell membrane, microvilli, mitochondria, nuclei and cytolysis of gut epithelial cells was observed.
Keywords
Culex pipiens; Punica granatum; larvicidal; Pomegranate peel; Midgut epithelium; histopathology
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