Phylogenetic relationships and taxonomy of three species of family Lethrinidae in the Red Sea, Egypt | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Article 2, Volume 22, Issue 1, January 2018, Pages 17-24 PDF (522.14 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejabf.2018.7710 | ||
| Authors | ||
| Abdelmeneam B. E. B. E.1; Ahmed, M. I. Ahmed, M. I.2; Madkour F. F. Madkour F. F.2; Hanafy M. H. Hanafy M. H.2 | ||
| 1Marine Science Department, Faculty of Science, Port Said University, Port Said, Egypt | ||
| 2Marine Science Department, Faculty of Science, Suez Canal University, Ismailia, Egypt | ||
| Abstract | ||
| Species of family Lethrinidae are of a great commercial importance in Egypt. Recent researches proved that we are far from having a full list of the family species diversity in Egypt. The purpose of this study was to clarify the phylogeny of commercial fish species of family Lethrinidae in the Egyptian Red Sea. Three commercial species of Lethrinidae (Lethrinus mahsena, Lethrinus nebulosus and Monotaxis grandoculis) were obtained from northern Red Sea proper. Samples were identified based on morphological characters. Identification was confirmed using DNA barcoding technique. DNA extraction was performed using Phenol-chloroform extraction method. In PCR, About 655 bp were amplified from the 5' region of the COI gene from mitochondrial DNA, then successful amplifications were sequenced. By Combining our sequence results with sequences of some species of Family Lethrinidae submitted to the GenBank, phylogenetic trees were constructed using p-distance Neighbor-Joining method. Results indicated that the use of DNA barcoding technique for emperor fish and big eye bream identification was successful, not only between different species of the family, but also within species, indicating the possible presence of cryptic species complex and the possible discovery of new genetic diversity. Of the three examined species from the Egyptian coasts; Monotaxis grandoculis showed the lowest genetic differences. Lethrinus mahsena showed the highest variations. | ||
| Keywords | ||
| Lethrinidae; Phylogeny; DNA barcoding; Taxonomy; Red Sea | ||
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