CHANGES IN PROTEIN CONTENT OF ARGAS (PERSICARGAS) PERSICUS (ACARI: ARGASIDAE) OVARY AFTER TREATMENT WITH ALLIUM SATIVUM EXTRACT | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 4, Volume 47, Issue 2, August 2017, Pages 263-272 PDF (481.23 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2017.77770 | ||
| Authors | ||
| OLA H. ZYAAN; SHIMAA S. AHMED; MOHAMED A. ABDOU | ||
| Department of Entomology, Faculty of Science, Ain Shams University, Cairo 11566, Egypt. | ||
| Abstract | ||
| Argas (Persicargas) persicus is a parasitic feeder on blood of poultry birds. After each blood meal a female is capable of producing a large numbers of eggs, the hatched larva remains attached to its bird host for seven or more days and draw numerous bulky blood meals. The ovary is the main factory part for the oogenesis (production of eggs). The targeting treatment with ecofriendly acaricide is required to disrupt the normal physiology of the ovary. This study aimed to analyse and quantify total and fractionated protein in the ovary of A. persicus females treated with Allium sativum extracts from fresh garlic bulbs. Group of engorged females were topically treated with garlic extract at 1.44% concentration, and another group with water (control). All females were crossed independently with normal males. In order to analyze the proteins of the ovary, untreated females were dissected 0, 1, 2, 3, 4, 5, 6, 7, & 20 days after feeding (DAF), represented different physiological states of tick and the treated females were dissected 3, 4, 5, 6, 7, & 20 DAF. The ovaries were homogenated and subjected to standard total and fractionated protein techniques. The protein concentration in ovary was significantly higher (P<0.05) in controls than in treated ones. Fractionated protein showed five characteristic bands to vitellogenesis process in controls. They reached highest concentration level at 7th days after feeding (DAF). These five bands disappeared in the treated samples except for one band in the corresponding time. | ||
| Keywords | ||
| Argas (Persicargas) persicus; Allium sativum (garlic bulbs extracts); Vitellogenesis; Total protein; SDS-PAGE protein | ||
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