Salama, S., Shafei, S., El-Ebiary, E. (2005). Differentiation between virulent and avirulent strains of Pasteurella multocida using restriction endonuclease analysis. test, 15(2), 177-179. doi: 10.21608/jvmr.2005.77963
S. S. Salama; S. M. Shafei; Elham A. El-Ebiary. "Differentiation between virulent and avirulent strains of Pasteurella multocida using restriction endonuclease analysis". test, 15, 2, 2005, 177-179. doi: 10.21608/jvmr.2005.77963
Salama, S., Shafei, S., El-Ebiary, E. (2005). 'Differentiation between virulent and avirulent strains of Pasteurella multocida using restriction endonuclease analysis', test, 15(2), pp. 177-179. doi: 10.21608/jvmr.2005.77963
Salama, S., Shafei, S., El-Ebiary, E. Differentiation between virulent and avirulent strains of Pasteurella multocida using restriction endonuclease analysis. test, 2005; 15(2): 177-179. doi: 10.21608/jvmr.2005.77963

Differentiation between virulent and avirulent strains of Pasteurella multocida using restriction endonuclease analysis

Journal of Veterinary Medical Research
Article 28, Volume 15, Issue 2, March 2005, Pages 177-179    PDF (143.22 K)
Document Type: Original Article
DOI: 10.21608/jvmr.2005.77963
Authors
S. S. Salama; S. M. Shafei; Elham A. El-Ebiary
Central Laboratory for Evaluation of Veterinary Biologics, Abbassia, Cairo, Egypt
Abstract
In this study we used one reference Climson University (CU) strain and 3 virulent
field strains isolated from naturally infected chicken and identified as P. Multocida.
DNA was extracted from all strains and subjected to restriction endonuclease analysis, using EcoRI, HpaII and Hind III revealing that, great similarity between either the reference or local virulent field isolates. The obtained results indicated that the most differentiable restriction endonuclease enzyme was the Hind III, which showed different band patterns between different strains.
Keywords
Strains; virulent; avirulent; Pasteurella; endonuclease; multocida; analysis
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