Madbouly, H., Nour El-Dien, A., Abeer, B. (2005). Preparation of inactivated tissue culture rabies vaccine adjuvanted with natural immune stimulants. test, 15(2), 215-220. doi: 10.21608/jvmr.2005.77984
H. M. Madbouly; A. M. Nour El-Dien; Boseila A. H. Abeer. "Preparation of inactivated tissue culture rabies vaccine adjuvanted with natural immune stimulants". test, 15, 2, 2005, 215-220. doi: 10.21608/jvmr.2005.77984
Madbouly, H., Nour El-Dien, A., Abeer, B. (2005). 'Preparation of inactivated tissue culture rabies vaccine adjuvanted with natural immune stimulants', test, 15(2), pp. 215-220. doi: 10.21608/jvmr.2005.77984
Madbouly, H., Nour El-Dien, A., Abeer, B. Preparation of inactivated tissue culture rabies vaccine adjuvanted with natural immune stimulants. test, 2005; 15(2): 215-220. doi: 10.21608/jvmr.2005.77984

Preparation of inactivated tissue culture rabies vaccine adjuvanted with natural immune stimulants

Journal of Veterinary Medical Research
Article 35, Volume 15, Issue 2, March 2005, Pages 215-220    PDF (393.11 K)
Document Type: Original Article
DOI: 10.21608/jvmr.2005.77984
Authors
H. M. Madbouly1; A. M. Nour El-Dien2; Boseila A. H. Abeer2
1Deptartment of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
2National Organization for Drug Control and Research, Cairo, Egypt
Abstract
The challenge virus standard (CVS-11) strain of fixed rabies virus was propagated in weaning mice brains and in suckling mice brains. It was also propagated on baby hamster kidney cell line (BHK-21) with use of diethylaminoethyl-dextran  (DEAEDextran). These passages were titerated in 3-4 weeks old white Swiss mice using mouse inoculation test (MIT). The virus harvest was concentrated using zinc acetate method , inactivated by beta-propiolactone (BPL) and adjuvanted with combination of vitamin E and selenium. The prepared adjuvanted inactivated tissue culture rabies vaccine was subjected to quality control tests as safety, sterility and potency using National Institute of Health (NIH) test against reference vaccine.
Keywords
Tissue; vaccine; rabies; stimulants; natural immune; adjuvanted
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