ALSHEIKH, A., ALBARRAK, A., DAFFALLA, O., NOURELDIN, E., MOHAMMED, W., SHRWANI, K., HOBANI, Y., ZAMIM, M., ASSIRI, A. (2017). THE USE OF NESTED-PCR TO DETECT THE PRESENCE OF PLASMODIUM IN ANOPHELES ARABIENSIS IN JAZAN REGION, SAUDI ARABIA. test, 47(1), 151-158. doi: 10.21608/jesp.2017.78021
A. A. ALSHEIKH; A. A. ALBARRAK; O. M. DAFFALLA; E. M. NOURELDIN; W. S. MOHAMMED; K. J. SHRWANI; Y. A. HOBANI; M. M. ZAMIM; A. M. ASSIRI. "THE USE OF NESTED-PCR TO DETECT THE PRESENCE OF PLASMODIUM IN ANOPHELES ARABIENSIS IN JAZAN REGION, SAUDI ARABIA". test, 47, 1, 2017, 151-158. doi: 10.21608/jesp.2017.78021
ALSHEIKH, A., ALBARRAK, A., DAFFALLA, O., NOURELDIN, E., MOHAMMED, W., SHRWANI, K., HOBANI, Y., ZAMIM, M., ASSIRI, A. (2017). 'THE USE OF NESTED-PCR TO DETECT THE PRESENCE OF PLASMODIUM IN ANOPHELES ARABIENSIS IN JAZAN REGION, SAUDI ARABIA', test, 47(1), pp. 151-158. doi: 10.21608/jesp.2017.78021
ALSHEIKH, A., ALBARRAK, A., DAFFALLA, O., NOURELDIN, E., MOHAMMED, W., SHRWANI, K., HOBANI, Y., ZAMIM, M., ASSIRI, A. THE USE OF NESTED-PCR TO DETECT THE PRESENCE OF PLASMODIUM IN ANOPHELES ARABIENSIS IN JAZAN REGION, SAUDI ARABIA. test, 2017; 47(1): 151-158. doi: 10.21608/jesp.2017.78021

THE USE OF NESTED-PCR TO DETECT THE PRESENCE OF PLASMODIUM IN ANOPHELES ARABIENSIS IN JAZAN REGION, SAUDI ARABIA

Journal of the Egyptian Society of Parasitology
Article 18, Volume 47, Issue 1, April 2017, Pages 151-158    PDF (1.56 M)
Document Type: Original Article
DOI: 10.21608/jesp.2017.78021
Authors
A. A. ALSHEIKH1; A. A. ALBARRAK2; O. M. DAFFALLA1; E. M. NOURELDIN1; W. S. MOHAMMED1; K. J. SHRWANI1; Y. A. HOBANI1; M. M. ZAMIM1; A. M. ASSIRI3
1National Center for Vector-Borne Diseases, MoH-Jazan, Saudi Arabia.
2Minister Deputy for Public Health, MoH-Riyadh, Saudi Arabia.
3General Director of Saudi CDC.
Abstract
The present study was carried out in 26 villages at two Governates (Al-Khobah, and Haroob) in Jazan Region in Southwest Saudi Arabia to identify and detect the presence of Plasmodium in Anopheles arabiensis using nested-PCR technique.
An. Arabiensis was identified by PCR and it was the predominant Anopheles mosquito in all the collection sites. A total of 257 An. Arabiensis females were collected and two samples from two villages (Almuatan and Alsabkha) out of 107 (1.87%) female mosquitoes from Haroob Governorate were found positive for the sporozoites of Plasmodium falciparum. Similarly, 3 out of 150 (2%) female mosquito samples from Um-alkhameir, AL-Khobah Governorate, were also found positive. Around fourfold increase of the sporozoite rate (from 0.61 to 2.0%) in An. arabiensis in Al-Khobah Governorate
has been observed compared to the previous study of 2007-2008. The wide spread of An. arabiensis in Jazan region with >90% of the malaria cases caused by P. falciparum, along with infectivity rate ranges between 1.87 to 2.0% for P. falciparum in Al-Khobah and Haroob Governorates, suggests that P. falciparum is the most predominant malaria parasite and An. Arabiensis is a very efficient malaria vector in the region. It also suggests more in-depth researches on the ecology, behavior, and control of An. Arabiensis to promote area-specific control programs.
Keywords
Malaria; Jazan; Saudi Arabia; Plasmodium falciparum; PCR; Anopheles arabiensis
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