RESPONSE OF BITTER AND SWEET ALMOND TO SODIUM AZIDE AS CHEMICAL MUTAGENE | ||
| Sinai Journal of Applied Sciences | ||
| Article 5, Volume 5, Issue 1, April 2016, Pages 53-62 PDF (614 K) | ||
| DOI: 10.21608/sinjas.2016.78630 | ||
| Authors | ||
| Gehan Y. Ramadan* 1; Mohamed D. ElDeep1; Ibrahim M. Ibrahim2 | ||
| 1Dept. Plant Prod., Fac. Environ. Agric. Sci., Suez Canal Univ., Arish, Egypt. | ||
| 2Inst. Genetic Eng. and Biotechnol., Sadat City, Monofia Univ., Egypt. | ||
| Abstract | ||
| Embryos, Shoot tips and one node cuttings from bitter and sweet almond are excised; sterilized, and cultured on Murashige & Skoog (MS) 1962 medium. Sodium azide for both proliferated bitter and sweet almond rootstocks was used. The obtained results showed that sodium azide induced the best results for mutation. Using embryo was the best explants in enhancing plant length at 2.5mM for 60 min of sweet almond in second sub culture. Also the best values of leaves number, shoot number, shoot length and leave per shoot at 2 mM for 60 min and the minimum value of stomata number was in 2mM for 60 min with shoot. The lowest leaf area was found in 1mM for 60 min with embryo. | ||
| Keywords | ||
| Sodium azide; Chemical mutagene; bitter and sweet almond; in vitro stomata number | ||
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