PREDICTION OF THE APPARENT ILEAL DIGESTIBILITY OF DIETS CONTAINING CARAWAY SEED SIEVING FOR RABBITS BY ITS CHEMICAL COMPONENTS OR BY IN-VITRO ENZYMATIC ANALYSIS AND COMPARED IT WITH IN VIVO DIGESTIBILITY VALUES | ||
| Egyptian Journal of Nutrition and Feeds | ||
| Article 9, Volume 22, Issue 2, August 2019, Pages 329-345 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejnf.2019.79426 | ||
| Author | ||
| Azza M.M. Badr | ||
| Regional Center for Feed and Food, Agriculture Research Center, Giza, Egypt. | ||
| Abstract | ||
| The use of multiple-enzymatic in vitro methods has been proven to be a good alternative to chemical analyses to stimulate the digestive processes and to predict energy values with greater precision in rabbits. The basal experimental diet was formulated and pelleted to cover the nutrient requirements of rabbits according to NRC (1977). The experimental groups were classified as the following: R1 fed the basal diet and served as the control group (R1), and control containing 2%, or 4% or 6% caraway seeds sieving (CSS) for R2, R3 and R4, respectively. The prediction equations of the DMD %, GEd% and DE (MJ Kg-1 DM) of diets using in vitro analysis, the highly significant (P<0.01) prediction effect of DMD was recorded with R3 (containing 4% CSS), the values were 76.60% in R3, followed 75.04% in R4 and the lowest value 73.89% in R2 compared with control 72.07%. Predicting nutritional values by analyzing the laboratory in vitro with the chemistry analysis (g / kg) of the dry matter, the higher significant (P<0.01) prediction of DMD% was recorded with R3, the values were 82.04% in R3, 80.37% in R4 and 79.14% in R2 compared with control 75.62%, the estimating GEd%, and. DE (MJ Kg-1DM) increased the prediction ratio for GEd% and DE (MJ / kg DM) using DMDinv and EE with R3, and the values were 82.81% and 14.07(MJ/kg DM) respectively. These values were higher than these predicted by in vitro only DMD in vivo trails were slightly higher values compared with DMD in vitro by 0.54%,0.38, 6.65 and 4.21% for R1, R2, R3, and R4, respectively. The prediction DMSin vivo from the equation in vitro system higher compared with the values DMDinv and values DMDin vivo trials, the values were 79.16%, 81.54 %,80.17%, and 76.28%, for R2, R3, and R4, respectively. Values of nitrogen-corrected apparent metabolizable energy content (AMEn) and its proportion on total gross energy (AMEn/GE%) in the diets CSS were highest values determined by chemical composition results compared with the same values obtained by using in vitro dry matter digestibility results. The highest significant values of AMEn/GE% and AMEn (kcal kg–1 DM) were obtained with R3 they were 76.20 and 3,460 kcal kg–1 DM, respectively. The values highest increased AMEn/GE% determined by using in vitro dry matter digestibility result was recorded with R3 by 9.48%, followed R4 by 6.66%, finally, R 2 by 4.97%, the values of AMEn (kcal kg–1 DM) were 2,903, 2,852 and 2,820, for R3, R4 and R2 compared with R1 control were 2,726. The Prediction apparent ileal digestibility of protein values of In-vitro was given the highest value compared to the digested protein by the digestion experience on rabbits. Results of endogenous protein losses (EPLg / kg DM intake) were lower with diets containing caraway seed sieving compared to the control diet. The values were 29.43, 27.60, 28.65% and R2, R3 and R4, respectively compared to the control 31.64%. Predicting the values of essential and non-essential amino acids by the results of the digested protein obtained from the in vitro enzyme system. A significant increase was recorded with rabbits’ diets containing caraway seed sieve by levels 2 and 4% for R2 and R3, respectively, but a nonsignificant increase recorded with the R4 which contains 6% compared with the control R1. Caraway seed saving containing medicines compounds of total antioxidant 319.77 mg/100g ascorbic acid equivalent, total flavonoids 120.56 m/100g Quercetin equivalent, total phenols 447 mg/100g gallic acid equivalent and carvacrol 3934 g/L. In conclusion, a study of diets containing 4% caraway seeds sieving (CSS) indicated the highest nutritional values. The nutritional values can be predicted through the estimation of in vitro dry matter enzymatic digestibility in rabbit diets. | ||
| Keywords | ||
| Caraway seed sieving; in Vitro; in vivo; prediction of AMEn/GE and AMEn; growth energy; digestible dry; organic matter; predicted amino acids | ||
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