Nashaat, Y., Hassan, S., Labib, A., Elaasar, M., Negm, A. (2018). COMPARATIVE STUDY OF THE CYTOTOXICTY AND GENOTOXICITY OF SILVER ZEOLITE AND NANO SILVER. test, 64(Issue 4 - October (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics)), 3995-4002. doi: 10.21608/edj.2018.79515
Yousra Mohamed Nashaat; Soha A. Hassan; Ahmed hussien Labib; Mostafa shaker Elaasar; Ahmed Maged Negm. "COMPARATIVE STUDY OF THE CYTOTOXICTY AND GENOTOXICITY OF SILVER ZEOLITE AND NANO SILVER". test, 64, Issue 4 - October (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics), 2018, 3995-4002. doi: 10.21608/edj.2018.79515
Nashaat, Y., Hassan, S., Labib, A., Elaasar, M., Negm, A. (2018). 'COMPARATIVE STUDY OF THE CYTOTOXICTY AND GENOTOXICITY OF SILVER ZEOLITE AND NANO SILVER', test, 64(Issue 4 - October (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics)), pp. 3995-4002. doi: 10.21608/edj.2018.79515
Nashaat, Y., Hassan, S., Labib, A., Elaasar, M., Negm, A. COMPARATIVE STUDY OF THE CYTOTOXICTY AND GENOTOXICITY OF SILVER ZEOLITE AND NANO SILVER. test, 2018; 64(Issue 4 - October (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics)): 3995-4002. doi: 10.21608/edj.2018.79515

COMPARATIVE STUDY OF THE CYTOTOXICTY AND GENOTOXICITY OF SILVER ZEOLITE AND NANO SILVER

Egyptian Dental Journal
Article 49, Volume 64, Issue 4 - October (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics), October 2018, Pages 3995-4002    PDF (897.09 K)
Document Type: Original Article
DOI: 10.21608/edj.2018.79515
Authors
Yousra Mohamed Nashaat1; Soha A. Hassan2; Ahmed hussien Labib3; Mostafa shaker Elaasar4; Ahmed Maged Negm5
1Associate Professor of Endodontics, October 6 University
2Associate Professor of Cell Biology, October 6 Universit
3Associate Professor of Endodontics Tanata University
4Lecturer of Endodontics; October 6 University
5Lecturer of Endosontics; Ahram Canadian University
Abstract
Silver ions and Nano silver particles have a great importance as they used as root canal sealers due to their antimicrobial properties. However biocompatibility and satisfactory physico-chemical properties should be achieved to directly contact the root canal sealers with the surrounding tissues. This study aimed to compare the cytotoxicity and genotoxicity of silver-zeolite with silver nanoparticles. MRC-5 cell line was used as an in vitro model. The cell viability was assessed using MTT cytotoxicity assay. Genotoxicity was tested by alkaline single cell gel electrophoresis (comet assay) for measuring DNA damage. Each material was tested in different concentrations (100mg/ml, 50mg/ml, 25mg/ml, 12.5mg/ml, and 6.25mg/ml). The results showed that silver zeolite has lower cytotoxic effect than silver nanoparticles among all different concentrations used in the study. Also the results showed unaccepted cytotoxicity levels for silver nanoparticles on concentrations of 100mg/ml and 50mg/ml. Data obtained from the Comet assay indicated that both silver nanoparticles and silver zeolite causes DNA damage with no significant difference between the two treated groups
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