Mostafa, H., Shehata, F., Omar, S., Kawana, K. (2020). THE EFFECT OF AMOXICILLIN ON THE SECRETORY STAGE OF AMELOGENESIS IN RATS. test, 45(1), 34-38. doi: 10.21608/adjalexu.2020.79929
Hossam M. Mostafa; Fathy I. Shehata; Samia O. Omar; Khadiga Y. Kawana. "THE EFFECT OF AMOXICILLIN ON THE SECRETORY STAGE OF AMELOGENESIS IN RATS". test, 45, 1, 2020, 34-38. doi: 10.21608/adjalexu.2020.79929
Mostafa, H., Shehata, F., Omar, S., Kawana, K. (2020). 'THE EFFECT OF AMOXICILLIN ON THE SECRETORY STAGE OF AMELOGENESIS IN RATS', test, 45(1), pp. 34-38. doi: 10.21608/adjalexu.2020.79929
Mostafa, H., Shehata, F., Omar, S., Kawana, K. THE EFFECT OF AMOXICILLIN ON THE SECRETORY STAGE OF AMELOGENESIS IN RATS. test, 2020; 45(1): 34-38. doi: 10.21608/adjalexu.2020.79929

THE EFFECT OF AMOXICILLIN ON THE SECRETORY STAGE OF AMELOGENESIS IN RATS

Alexandria Dental Journal
Article 6, Volume 45, Issue 1, April 2020, Pages 34-38    PDF (289.33 K)
Document Type: Original Article
DOI: 10.21608/adjalexu.2020.79929
Authors
Hossam M. Mostafa* 1; Fathy I. Shehata2; Samia O. Omar2; Khadiga Y. Kawana2
1Master of science degree in Oral Biology 2015, Alexandria University
2Professor of Oral Biology, Faculty of Dentistry, Alexandria University.
Abstract
Introduction: Molar–incisor hypomineralization is a developmental enamel defect affecting first permanent molars and incisors. The intake of antibiotics during pregnancy and early childhood has been associated with molar incisor hypomineralization.
Objectives: Was to determine the effect of amoxicillin on the secretory a stage of amelogenesis histologically and histomorphometrically.
Materials and methods: Fourteen pregnant rats were randomly assigned to two groups that received physiological solution (control group), 250 mg/kg/day amoxicillin (study group). Directly after birth, fourteen pups representing each group received the same treatment with dose equivalent to their body weight then they were euthanized at day 7 birth. The ameloblasts of developing molars were examined histologically and histomorphometrically.
Results:In day 7 after birth, the ameloblasts in control group revealed normal features of amelogenesis while in study group, numerous vacuolar like structures were seen in between secretory ameloblasts.
Conclusions: Amoxicillin disrupts the normal structure of enamel and impairs the secretion of enamel matrix
Keywords
Ameloblast; amoxicillin; secretory; amelogenesis
References
  1. Weerheijm K, Jälevik B, Alaluusua S. Molar–incisor hypomineralisation. Caries Res. 2001;35:390-1.
  2. Alaluusua S. Aetiology of molar-incisor hypomineralisation: a systematic review. Eur Arch Paediatr Dent. 2010;11:53-8.
  3. Taylor GD. Molar incisor hypomineralisation. EBD. 2017;18:15.
  4. Silva MJ, Scurrah KJ, Craig JM, Manton DJ, Kilpatrick N. Etiology of molar incisor hypomineralization–A systematic review. Community Dent Oral Epidemiol. 2016;44:342-53.
  5. Pitiphat W, Luangchaichaweng S, Pungchanchaikul P, Angwaravong O, Chansamak N. Factors associated with molar incisor hypomineralization in T hai children. Eur J Oral Sci. 2014;122:265-70.
  6. Hu JC-C, Chun Y-HP, Al Hazzazzi T, Simmer JP. Enamel formation and amelogenesis imperfecta. CTO. 2007;186:78-85.
  7. Nanci A, Ahluwalia JP, Pompura JR, Smith CE. Biosynthesis and secretion of enamel proteins in the rat incisor. Anat Rec. 1989;224:277-91.
  8. DenBesten P, Li R. Characterization of amelogenin mRNA from secretory-and maturation-stage rat incisor enamel. Arch Oral Biol. 1992;37:1097-100.
  9. Smith C. Cellular and chemical events during enamel maturation. Crit Rev Oral Biol Med. 1998;9:128-61.
  10. Sabandal MM, Schäfer E. Amelogenesis imperfecta: review of diagnostic findings and treatment concepts. Odont. 2016;104:245-56.
  11. Oscáriz JC, Pisabarro AG. Classification and mode of action of membrane-active bacteriocins produced by grampositive bacteria. Int Microbiol. 2001;4:13-9.
  12. Kohanski MA, Dwyer DJ, Collins JJ. How antibiotics kill bacteria: from targets to networks. Nat Rev Microbiol. 2010;8:423.
  13. Essack SY. The development of β-lactam antibiotics in response to the evolution of β-lactamases. Pharm Res. 2001;18:1391-9.
  14. Hansen JL, Ippolito JA, Ban N, Nissen P, Moore PB, Steitz TA. The structures of four macrolide antibiotics bound to the large ribosomal subunit. Mol Cell. 2002;10:117-28.
  15. Dagan R, Hoberman A, Johnson C, Leibovitz EL, Arguedas A, Rose FV, et al. Bacteriologic and clinical efficacy of high dose amoxicillin/clavulanate in children with acute otitis media. Pediatr Infect Dis J. 2001;20:829-37.
  16. De Souza JF, Gramasco M, Jeremias F, Santos‐Pinto L, Giovanini AF, Cerri PS, et al. Amoxicillin diminishes the thickness of the enamel matrix that is deposited during the secretory stage in rats. Int J Paediatr Dent. 2016;26:199-210.
  17. Council NR. Guide for the care and use of laboratory animals: NAP; 2010.
  18. Laisi S, Ess A, Sahlberg C, Arvio P, Lukinmaa P-L, Alaluusua S. Amoxicillin may cause molar incisor hypomineralization. J Dent Res. 2009;88:132-6.
  19. Hong L, Levy SM, Warren JJ, Dawson DV, Bergus GR, Wefel JS. Association of amoxicillin use during early childhood with developmental tooth enamel defects. Arch Pediatr Adolesc Med. 2005;159:943-8.
  20. Souza J, Costa-Silva C, Jeremias F, Santos-Pinto L, Zuanon ACC, Cordeiro RdCL. Molar incisor hypomineralisation: possible aetiological factors in children from urban and rural areas. Eur Arch Paediatr Dent. 2012;13:164-70.
  21. Sahlberg C, Pavlic A, Ess A, Lukinmaa P-L, Salmela E, Alaluusua S. Combined effect of amoxicillin and sodium fluoride on the structure of developing mouse enamel in vitro. Arch Oral Biol. 2013;58:1155-64.
  22. Kumazawa K, Sawada T, Yanagisawa T, Shintani S. Effect of single-dose amoxicillin on rat incisor odontogenesis: a morphological study. Clin Oral Invest. 2012;16:835-42.
  23. Muñoz CS, Silva AP, Solano F, Castells MT, Vicente A, Ruiz AJO. Effect of antibiotics and NSAIDs on cyclooxygenase-2 in the enamel mineralization. Sci Rep. 2018;8:4132.
  24. Westergaard J. Structural changes induced by tetracycline in secretory ameloblasts in young rats. Scand J Dent Res. 1980;88:481
Statistics
Article View: 391
PDF Download: 838