Radioiodination of Cefoperazone with 125I and its Biological Distribution in Mice | ||
| Arab Journal of Nuclear Sciences and Applications | ||
| Article 7, Volume 51, Issue 3, July 2018, Pages 62-68 PDF (271.17 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajnsa.2017.1983.1014 | ||
| Authors | ||
| Dina Moustafa El-Sharawy* 1; sabah khater2; marwa el refaay2 | ||
| 1Cyclotron project, Atomic energy authority | ||
| 2cyclotron project, Atomic energy authority | ||
| Abstract | ||
| Cefoperazone tracer was radioiodinated with 125I by replacing the hydrogen atom with iodonium cation I+ which is known as an electrophilic substitution reaction. All the reaction parameters were studied to produce maximum Radiochemical yield (RCY) of 125I-Cefoperazone [125I-Cefo] by using 3.7 MBq of Na125I, 150 µg of cefoperazone as substrate, 100 µg of Chloramine-T as oxidizing agent in water at 60ºC for 20 min, the RCY of 125I-Cefo up to 90.2% The radiochemical yield was determined practically by electrophoresis 0.02M phosphate buffer with pH 7. A theoretical study was performed using Gaussian 09 to calculate the heat of formation of the expected products. The tracer was separated and purified by high-pressure liquid chromatography (HPLC) with phosphate buffer pH 6.8: methanol (3:1 v/v). The biological distribution of the tracer was studied in normal and infected mice with E-coli bacteria and sacrificed after different time’s intervals activity in each organ was counted and expressed as a percentage of the injected activity per organ. | ||
| Keywords | ||
| Cefoperazone; Radioiodination; Gaussian 09; RCY | ||
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