Kolawole,, O., Ibitayo, A., Mustapha, S., Olubayode, A. (2020). The Incidence of Vibrio Cholerae as an Indicator of Pollution of Oyun River in Ilorin, Kwara State Nigeria. test, 12(1), 45-54. doi: 10.21608/eajbsg.2020.82018
Olatunji M. Kolawole,; Anibijuwon, Ibikunle Ibitayo; Suleiman M. Mustapha; Asaolu Olubayode. "The Incidence of Vibrio Cholerae as an Indicator of Pollution of Oyun River in Ilorin, Kwara State Nigeria". test, 12, 1, 2020, 45-54. doi: 10.21608/eajbsg.2020.82018
Kolawole,, O., Ibitayo, A., Mustapha, S., Olubayode, A. (2020). 'The Incidence of Vibrio Cholerae as an Indicator of Pollution of Oyun River in Ilorin, Kwara State Nigeria', test, 12(1), pp. 45-54. doi: 10.21608/eajbsg.2020.82018
Kolawole,, O., Ibitayo, A., Mustapha, S., Olubayode, A. The Incidence of Vibrio Cholerae as an Indicator of Pollution of Oyun River in Ilorin, Kwara State Nigeria. test, 2020; 12(1): 45-54. doi: 10.21608/eajbsg.2020.82018

The Incidence of Vibrio Cholerae as an Indicator of Pollution of Oyun River in Ilorin, Kwara State Nigeria

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Article 5, Volume 12, Issue 1, June 2020, Pages 45-54    PDF (690.67 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2020.82018
Authors
Olatunji M. Kolawole,1; Anibijuwon, Ibikunle Ibitayo2; Suleiman M. Mustapha1; Asaolu Olubayode2
11-Department of Microbiology, Faculty of Life Sciences, University of Ilorin, Nigeria. 2-Institute of Molecular Science and Biotechnology, University of Ilorin, Nigeria
2Department of Microbiology, Faculty of Life Sciences, University of Ilorin, Nigeria
Abstract
Bacteria, which are the most common pathogens in water, gain entrance into water mostly through fecal contamination and this poses significant risks to human and animal health. The causative agent of cholera is globally autochthonous to the aquatic environment and it’s not confined to only cholera endemic areas. This study is a survey of the microbiological quality of Oyun River and most importantly using the detection and incidence of Vibrio cholerae as an additional indicator of pollution of faecal origin. Molecular characterization and confirmation of bacterial isolates were done via Polymerase Chain Reaction (PCR) and DNA sequencing. The microbiological and molecular analysis revealed the presence of E. coli, Streptococcus thermophillusTH1435, Vibrio Parahaemolyticus wp_051483013, Enterobacter cloacae strain sugR_1, Vibrio campbellii HYO1 Vibrio cholerae and Thermobaculum terenum in the Oyun River. Further sequence analysis revealed the presence of six new strains of enteric bacteria which was allocated the following accession numbers after deposition at the GenBank (MT275484; MT275485; MT275486; MT275487; MT275488; MT275489). The confirmation of isolates from the Vibrio genus and other enteric bacteria posits that the pollution is of faecal origin and thus, adequate monitoring to control activities along the riverside should be upheld by both the citizens and government authorities to avoid preventable outbreaks. 
Keywords
Enteric bacteria; Vibrio cholerae; Aquatic; Oyun River; Faecal
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