Azab, N., Salim, R., Darwish, M. (2017). Study of thrombophilic gene polymorphisms in Egyptian patients with deep venous thrombosis using in situ hybridization technique.. test, 37(1), 18-35. doi: 10.21608/besps.2017.8221
Naglaa Ibrahim Azab; Rabab Fawzy Salim; Mohamed Saeed Darwish. "Study of thrombophilic gene polymorphisms in Egyptian patients with deep venous thrombosis using in situ hybridization technique.". test, 37, 1, 2017, 18-35. doi: 10.21608/besps.2017.8221
Azab, N., Salim, R., Darwish, M. (2017). 'Study of thrombophilic gene polymorphisms in Egyptian patients with deep venous thrombosis using in situ hybridization technique.', test, 37(1), pp. 18-35. doi: 10.21608/besps.2017.8221
Azab, N., Salim, R., Darwish, M. Study of thrombophilic gene polymorphisms in Egyptian patients with deep venous thrombosis using in situ hybridization technique.. test, 2017; 37(1): 18-35. doi: 10.21608/besps.2017.8221

Study of thrombophilic gene polymorphisms in Egyptian patients with deep venous thrombosis using in situ hybridization technique.

Bulletin of Egyptian Society for Physiological Sciences
Article 2, Volume 37, Issue 1, June 2017, Pages 18-35    PDF (839.15 K)
Document Type: Original Article
DOI: 10.21608/besps.2017.8221
Authors
Naglaa Ibrahim Azab* 1; Rabab Fawzy Salim1; Mohamed Saeed Darwish2
1Department of Medical Biochemistry, Faculty of Medicine, Benha University, Benha - Egypt.
2Department of Cardiovascular medicine, Faculty of Medicine, Benha University, Benha - Egypt.
Abstract
Up to 70% of thrombotic patients with no identifiable risk factors were termed idiopathic. Now, molecular diagnostics combined with existing laboratory techniques allow accurate classification of at least half of patients with inherited thrombotic disorders. However, the previously studied genetic variants explain only a fraction of all thromboembolic events, so the aim of the present study was to expand the genetic prevalence determination to include also more extensive thrombophilic gene polymorphisms in patients with DVT compared to healthy subjects in Egyptian population. This study was conducted on 75 patients with DVT and 45 age and sex matched healthy subjects as control group. The diagnosis of DVT was based on patient’s history, clinical findings, D-dimer test, and confirmed by Doppler ultrasonography. Both groups were assessed for thrombophilic gene polymorphisms using multiplex polymerase chain reaction and reverse-hybridization technique through CVD strip assay. It was found that FV Leiden G1691A (P=0.001), Factor V H1299R (P=0.02), MTHFR A1298C (P=0.02), β-fibrinogen−455 G/A (P=0.01), PAI-1 4G/5G (P=0.03) and ACE (P=0.03) polymorphisms were all significantly associated with an increased risk of DVT. Our data are of extreme importance in clinical practice for introducing the extended CVD panel into routine molecular diagnostic tests to allow management of thrombotic patients and screening, thromboprophylaxis and genetic counselling for high-risk individuals.
Keywords
Venous Thromboembolism; Deep Venous Thrombosis; Genetic risk factors; Thrombophilic gene polymorphisms; Cardiovascular disease strip assay
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