Growth Kinetics and Metronidazole Sensitivity of Blastocystis Sp. Isolated from Colorectal Carcinoma (CRC) and Non-CRC Patients | ||
| The Egyptian Journal of Hospital Medicine | ||
| Article 29, Volume 72, Issue 1, July 2018, Pages 3850-3856 PDF (446.8 K) | ||
| Document Type: Original Article | ||
| Abstract | ||
| Blastocystis sp. is the most common protist detected in human fecal samples in numerous studies globally. Extreme debate regarding Blastocystis sp. pathogenicity exists. Some studies have speculated a possible correlation between Blastocystis and CRC. No previous study has investigated the presence of non-genotypic differences in Blastocystis sp. isolated from CRC and non-CRC patients. The present work aimed to investigate the growth kinetics (G.K) and metronidazole (MTZ) sensitivity in Blastocystis isolates from CRC and apparent non-CRC whether symptomatic and asymptomatic Blastocystis carriers. Seven isolates from CRC patients and 6 from symptomatic and 6 from asymptomatic non-CRC carriers were cultured in Locke's Egg (LE) medium supplemented with bovine serum and antibiotic mixture and incubated at 37◦ C. Mean viable organisms counts of each isolate were counted every 24 hours in medium to follow their GK and after exposure to different MTZ concentrations to perform drug sensitivity assay. In vitro growth kinetics of CRC and Non-CRC symptomatic isolates were nearly similar with higher peaks attained at 72 hours incubation than by the slower growing non-CRC-asymptomatic isolates. MTZ-sensitivity of CRC isolates was nearly similar to that of non-CRC asymptomatic isolates, both were significantly more sensitive than the symptomatic isolates especially at high drug concentration of 200 μg/ml of the medium. | ||
| Keywords | ||
| Blastocystis sp; Colorectal carcinoma; Symptomatic; Asymptomatic; growth kinetics; metronidazole sensitivity | ||
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