HH, Z., BM, L., DA, S., OE, M., RM, S. (2015). GENOTOXICITY OF FORMALDEHYDE IN PERIPHERAL BLOOD LYMPHOCYTES AMONG PATHOLOGY LABORATORY WORKERS. test, 39(2), 243-254. doi: 10.21608/ejom.2015.826
Zayet HH; Lotfy BM; Sabry DA; Mohamed OE; Said RM. "GENOTOXICITY OF FORMALDEHYDE IN PERIPHERAL BLOOD LYMPHOCYTES AMONG PATHOLOGY LABORATORY WORKERS". test, 39, 2, 2015, 243-254. doi: 10.21608/ejom.2015.826
HH, Z., BM, L., DA, S., OE, M., RM, S. (2015). 'GENOTOXICITY OF FORMALDEHYDE IN PERIPHERAL BLOOD LYMPHOCYTES AMONG PATHOLOGY LABORATORY WORKERS', test, 39(2), pp. 243-254. doi: 10.21608/ejom.2015.826
HH, Z., BM, L., DA, S., OE, M., RM, S. GENOTOXICITY OF FORMALDEHYDE IN PERIPHERAL BLOOD LYMPHOCYTES AMONG PATHOLOGY LABORATORY WORKERS. test, 2015; 39(2): 243-254. doi: 10.21608/ejom.2015.826

GENOTOXICITY OF FORMALDEHYDE IN PERIPHERAL BLOOD LYMPHOCYTES AMONG PATHOLOGY LABORATORY WORKERS

Egyptian Journal of Occupational Medicine
Article 8, Volume 39, Issue 2, July 2015, Pages 243-254    PDF (166.83 K)
Document Type: Study paper
DOI: 10.21608/ejom.2015.826
Authors
Zayet HH1; Lotfy BM1; Sabry DA2; Mohamed OE3; Said RM1
1Department of Occupational and Environmental Medicine
2Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University
3Human Cytogenetics, National Research Center
Abstract
Introduction: Formaldehyde (FA) is a chemical, traditionally used in pathology and anatomy laboratories as a tissue preservative. Many studies clearly indicated that FA can induce genotoxic effects in peripheral blood lymphocytes. Aim of work: to evaluate the genotoxic effects of formaldehyde among medical and paramedical personnel in The Histopathology Laboratory. Materials and Methods: The study was conducted in histopathology laboratory including 30 workers occupationally exposed to formaldehyde and 29 persons from other lab in kasr Al Aini hospital matching the exposed group for age, sex and socioeconomic status with no history of occupational exposure to formaldehyde. A questionnaire was done including inquiries about age, sex, occupational history and special habits. All participants were subjected to the following laboratory investigations: measuring DNA protein crosslink (DPC), sister chromatid exchange (SCE) and micronucleus (Mn) frequency in peripheral blood lymphocytes. Environmental monitoring of the work place was done to measure the level of FA at different place in the pathology lab. Results: This study showed statistically significant increase of DPC with significant increase in sister chromatid exchange (SCE) and micronucleus MN in peripheral blood lymphocytes. Conclusion: The studied group was exposed to formaldehyde level above the exposure limits recommended by NIOSH, ACGIH and OSHA with adverse health effects in the form of increase in DPC, SCE and Mn as genotoxicity biomarkers. These genotoxicity biomarkers are considered to play an important role in the carcinogenesis of FA.
 
Keywords
Formaldehyde; Environmental monitoring; Sister chromatide exchange; micronucleus; DNA protein crosslink
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