The possible ameliorative role of mesenchymal stem cells and curcumin on the lung in experimentally induced type I diabetes: Histological and immunohistochemical study | ||
| Journal of Medical Histology | ||
| Article 7, Volume 3, Issue 1, June 2019, Pages 102-114 PDF (1.35 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jmh.2019.15404.1060 | ||
| Authors | ||
| Amany Hamoud* 1; Hala Metwally2 | ||
| 1Departments of Anatomy and Embryology Faculty of Medicine,Cairo University, Cairo, Egypt | ||
| 2Departments of Clinical Pathology, Faculty of Medicine,Cairo University, Cairo, Egypt | ||
| Abstract | ||
| Background: Type 1 diabetes mellitus affects lungs due to poor glycemic control. Improvement in both lung function and architecture in experimentally-induced lung injury that received exogenous AMSCs by different mechanisms was found. Curcumin has a potent antioxidant and antifibrotic function. Objective: The current study was designed to elucidate the possible ameliorative role of mesenchymal stem cells and curcumin on the lung in experimentally induced type I diabetes by histological and immunohistochemical study. Materials and Methods:Twenty six adult albino rats were divided into five groups: donor, control group, curcumin group and adipose derived mesenchymal stem cell (AMSCs) group. Group II (diabetic group) received a single dose STZ 50 mg/kg/day, group III received STZ and powdered diet supplemented with 0.05% curcumin, group IV received STZ and 1x106 of cultured and labelled rat AMSCs. Lung sections were subjected to histological, morphometric, biochemical and statistical studies. Results: Diabetes induced histological changes in lung in the form of inflammatory changes in bronchiole, a sig increase in the thickness of interalveolar septa, thickness of the vessels wall, area % of collagen and count CD105+ve cells. Sig decrease in antioxidant activity and sig increase in oxidant enzymes. Sig increase in L-hydroxyproline(L-hyp) level as well STAT and SMAD genes. Administration of curcumin and AMSCs improved sig the previous changes. Conclusion: Diabetes induced lung injuries in rats that was ameliorated by by curcumin, but AMSCs have more pronounced effect than curcumin therapy. | ||
| Keywords | ||
| ADMSCs; antioxidant; curcumin; diabetes; lung | ||
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